System and Method For Detecting Multiple Molecules in One Assay
First Claim
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1. A system for performing an assay on a sample, the system comprising:
- a cartridge havinga planar waveguide with upper and lower planar surfaces defining an optical axis therebetween, the optical axis being perpendicular to a normal vector of the upper and lower surfaces, the upper planar surface having a plurality of capture molecules bound thereto,an integral portion, coupled to the planar waveguide, for focusing a light beam, propagating parallel to but offset from the optical axis, to the planar waveguide such that focusing light within the integral lens portion and incident on the upper planar surface has a non-zero, internal propagation angle β
relative to the upper planar surface, anda sample chamber for receiving and containing the sample such that the sample comes into contact with the plurality of capture molecules; and
a reader instrument includinga receiving mechanism for positioning the cartridge therein,the light source for providing the light beam at an angle parallel to the optical axis,a detector for detecting a light signal from a portion of the planar surface on which the plurality of capture molecules is bound, andan analysis module for receiving and analyzing the light signal from the detector.
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Abstract
A rapid diagnostic system that delivers a panel of serologic assay results using a small amount of blood, serum, or plasma is described. The system includes a disposable cartridge, including an integral lens portion coupled to a planar waveguide, and a reader instrument, based on planar waveguide imaging technology. The cartridge incorporates a microarray of recombinant antigens and antibody controls in a fluidic channel, providing multiple parallel fluorescence assay results for a single sample.
22 Citations
20 Claims
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1. A system for performing an assay on a sample, the system comprising:
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a cartridge having a planar waveguide with upper and lower planar surfaces defining an optical axis therebetween, the optical axis being perpendicular to a normal vector of the upper and lower surfaces, the upper planar surface having a plurality of capture molecules bound thereto, an integral portion, coupled to the planar waveguide, for focusing a light beam, propagating parallel to but offset from the optical axis, to the planar waveguide such that focusing light within the integral lens portion and incident on the upper planar surface has a non-zero, internal propagation angle β
relative to the upper planar surface, anda sample chamber for receiving and containing the sample such that the sample comes into contact with the plurality of capture molecules; and a reader instrument including a receiving mechanism for positioning the cartridge therein, the light source for providing the light beam at an angle parallel to the optical axis, a detector for detecting a light signal from a portion of the planar surface on which the plurality of capture molecules is bound, and an analysis module for receiving and analyzing the light signal from the detector. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A method for performing an assay on a sample, the method comprising:
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providing a cartridge, which cartridge includes a planar waveguide with upper and lower planar surfaces defining an optical axis therebetween, the optical axis being perpendicular to a normal vector of the upper and lower surfaces, the upper planar surface having a plurality of capture molecules bound thereto, an integral lens portion, coupled to the planar waveguide, for focusing a light beam, propagating parallel to but offset from the optical axis, to the planar waveguide such that focusing light within the integral lens portion and incident on the upper planar surface has a non-zero, internal propagation angle β
relative to the upper planar surface, anda sample chamber for receiving and containing the sample such that the sample comes into contact with the plurality of capture molecules; introducing the sample into the sample chamber of the cartridge; providing a reader instrument, which reader instrument includes a receiving mechanism for positioning the cartridge therein, the light source for providing the light beam, a detector for detecting a light signal from a portion of the planar surface on which the plurality of capture molecules is bound, and an analysis module for receiving and analyzing the light signal from the detector; inserting the cartridge, containing the sample, into the reader instrument; using the light source, illuminating a portion of the planar waveguide, via refraction of the light beam by the integral lens portion, at which the plurality of capture molecules are bound such that, if the sample includes a target analyte, the target analyte interacts with the plurality of capture molecules so as to produce a light signal; capturing the light signal; and analyzing the light signal. - View Dependent Claims (15, 16, 17, 18, 19, 20)
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Specification