SYSTEM AND METHOD FOR PROCESSING AND DETECTING NUCLEIC ACIDS
First Claim
1. A method for processing and detecting nucleic acids from a biological sample, with a cartridge including a fluidic pathway coupled to a sample port and a reagent port, wherein the method comprises:
- dispensing the biological sample into the fluidic pathway through the sample port of the cartridge;
separating a volume of nucleic acids from the biological sample within a first portion of the fluidic pathway;
removing at least a portion of the volume of nucleic acids from the fluidic pathway through the reagent port or the sample port of the cartridge;
combining the portion of the volume of nucleic acids with a molecular diagnostic reagent to produce a nucleic acid-reagent mixture; and
delivering the nucleic acid-reagent mixture through a second portion of the fluidic pathway to a detection chamber.
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Accused Products
Abstract
A system and method for processing and detecting nucleic acids from a set of biological samples, comprising: a capture plate and a capture plate module configured to facilitate binding of nucleic acids within the set of biological samples to magnetic beads; a molecular diagnostic module configured to receive nucleic acids bound to magnetic beads, isolate nucleic acids, and analyze nucleic acids, comprising a cartridge receiving module, a heating/cooling subsystem and a magnet configured to facilitate isolation of nucleic acids, a valve actuation subsystem configured to control fluid flow through a microfluidic cartridge for processing nucleic acids, and an optical subsystem for analysis of nucleic acids; a fluid handling system configured to deliver samples and reagents to components of the system to facilitate molecular diagnostic protocols; and an assay strip configured to combine nucleic acid samples with molecular diagnostic reagents for analysis of nucleic acids.
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Citations
35 Claims
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1. A method for processing and detecting nucleic acids from a biological sample, with a cartridge including a fluidic pathway coupled to a sample port and a reagent port, wherein the method comprises:
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dispensing the biological sample into the fluidic pathway through the sample port of the cartridge; separating a volume of nucleic acids from the biological sample within a first portion of the fluidic pathway; removing at least a portion of the volume of nucleic acids from the fluidic pathway through the reagent port or the sample port of the cartridge; combining the portion of the volume of nucleic acids with a molecular diagnostic reagent to produce a nucleic acid-reagent mixture; and delivering the nucleic acid-reagent mixture through a second portion of the fluidic pathway to a detection chamber. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. A method for processing and detecting nucleic acids from a set of biological samples, comprising:
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combining each biological sample of the set of biological samples with a quantity of magnetic beads to produce a set of nucleic acid-magnetic bead samples; transferring substantially all of each nucleic acid-magnetic bead sample of the set of nucleic acid-magnetic bead samples to a corresponding fluidic pathway of a set of fluidic pathways; and detecting nucleic acids using a set of detection chambers coupled to the set of fluidic pathways. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28)
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29. A method for processing and detecting nucleic acids within a fluidic pathway including a set of occlusion positions, comprising:
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occluding the fluidic pathway at a first subset of the set of occlusion positions, thus defining a first truncated fluidic pathway passing through a magnetic field; capturing a sample of nucleic acids bound to magnetic beads within the first truncated fluidic pathway, by the magnetic field; and occluding the fluidic pathway at a second subset of the set of occlusion positions, thus defining a second truncated fluidic pathway containing the sample of nucleic acids bound to magnetic beads. - View Dependent Claims (30, 31, 32, 33, 34, 35)
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Specification