HUMAN G-PROTEIN CHEMOKINE RECEPTOR HSATU68

• US 20130210149A1
• Filed: 01/31/2011
• Published: 08/15/2013
• Est. Priority Date: 01/11/1996
• Status: Active Grant

• Current Assignee: Human Genome Sciences Incorporated (GlaxoSmithKline PLC)
• Original Assignee: Human Genome Sciences Incorporated (GlaxoSmithKline PLC)
• Inventors: Li, Yi
• Granted Patent: US 8,937,169 B2
• Time in Patent Office: Days
• US Class Current: 435/455
• CPC Class Codes: C07K 14/7158 for chemokines
• CPC Class Codes: C12Q 1/6883 for diseases caused by alterations of genetic material
• CPC Class Codes: G01N 33/6863 Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors

Abstract

Human G-protein chemokine receptor polypeptides and DNA (RNA) encoding such polypeptides and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed are methods for utilizing such polypeptides for identifying antagonists and agonists to such polypeptides and methods of using the agonists and antagonists therapeutically to treat conditions related to the underexpression and overexpression of the G-protein chemokine receptor polypeptides, respectively. Also disclosed are diagnostic methods for detecting a mutation in the G-protein chemokine receptor nucleic acid sequences and detecting a level of the soluble form of the receptors in a sample derived from a host.

Claims

1. An isolated nucleic acid molecule comprising a polynucleotide having a nucleotide sequence at least 95% identical to a sequence selected from the group consisting of:

(a) a polynucleotide fragment of SEQ ID NO;

1, SEQ ID NO;

3, or a polynucleotide fragment of the cDNA sequence contained in ATCC Deposit NO;

97334, which is hybridizable to SEQ ID NO;

1 or SEQ ID NO;

3,

(b) a polynucleotide encoding a polypeptide fragment of SEQ ID NO;

2, SEQ ID NO;

4, or a polypeptide fragment encoded by the cDNA sequence contained in cDNA ATCC Deposit NO;

97334, which is hybridizable to SEQ ID NO;

1 or SEQ ID NO;

3;

(c) a polynucleotide encoding a polypeptide fragment of a polypeptide encoded by SEQ ID NO;

1, SEQ ID NO;

3, or a polypeptide fragment encoded by the cDNA sequence contained in cDNA ATCC Deposit NO;

97334, which is hybridizable to SEQ ID NO;

1 or SEQ ID NO;

3;

(d) a polynucleotide encoding a polypeptide domain of SEQ ID NO;

2, SEQ ID NO;

4, or a polypeptide domain encoded by the cDNA sequence contained in cDNA ATCC Deposit NO;

97334, which is hybridizable to SEQ ID NO;

1 or SEQ ID NO;

3;

(e) a polynucleotide encoding a polypeptide epitope of SEQ ID NO;

2, SEQ ID NO;

4, or a polypeptide epitope encoded by the cDNA sequence contained in cDNA ATCC Deposit NO;

97334, which is hybridizable to SEQ ID NO;

1 or SEQ ID NO;

3;

(f) a polynucleotide encoding a polypeptide of SEQ ID NO;

2, SEQ ID NO;

4, or the cDNA sequence contained in cDNA ATCC Deposit NO;

97334, which is hybridizable to SEQ ID NO;

1 or SEQ ID NO;

3, having biological activity;

(g) a polynucleotide which is a variant of SEQ ID NO;

1 or SEQ ID NO;

3;

(h) a polynucleotide which is an allelic variant of SEQ ID NO;

1 or SEQ ID NO;

3;

(i) a polynucleotide which encodes a species homologue of SEQ ID NO;

2 or SEQ ID NO;

4; and

(j) a polynucleotide capable of hybridizing under stringent conditions to any one of the polynucleotides specified in (a)-(i), wherein said polynucleotide does not hybridize under stringent conditions to a nucleic acid molecule having a nucleotide sequence of only A residues or of only T residues.

2. The isolated nucleic acid molecule of claim 1, wherein the polynucleotide fragment comprises a nucleotide sequence encoding a protein.

3. The isolated nucleic acid molecule of claim 1, wherein the polynucleotide fragment comprises a nucleotide sequence encoding the sequence identified as SEQ ID NO:

2, SEQ ID NO;

4, or the polypeptide encoded by the cDNA sequence contained in cDNA ATCC Deposit NO;

97334, which is hybridizable to SEQ ID NO;

1 or SEQ ID NO;

3.

4. The isolated nucleic acid molecule of claim 1, wherein the polynucleotide fragment comprises the entire nucleotide sequence of SEQ ID NO:

1, the entire nucleotide sequence of SEQ ID NO;

3, or the cDNA sequence contained in cDNA ATCC Deposit NO;

97334, which is hybridizable to SEQ ID NO;

1 or SEQ ID NO;

3.

5. The isolated nucleic acid molecule of claim 2, wherein the nucleotide sequence comprises sequential nucleotide deletions from either the C-terminus or the N-terminus.

6. The isolated nucleic acid molecule of claim 3, wherein the nucleotide sequence comprises sequential nucleotide deletions from either the C-terminus or the N-terminus.

7. A recombinant vector comprising the isolated nucleic acid molecule of claim 1.

8. A method of making a recombinant host cell comprising:

(a) inserting the isolated nucleic acid of claim 1 into a vector;

(b) introducing the vector into a host cell.

9. A recombinant host cell produced by the method of claim 8.

10. The recombinant host cell of claim 9 comprising vector sequences.

11. -24. (canceled)