INCREASED ISOPRENE PRODUCTION USING THE ARCHAEAL LOWER MEVALONATE PATHWAY
2 Assignments
0 Petitions
Accused Products
Abstract
The invention features methods for producing isoprene from cultured cells using a feedback-resistant mevalonate kinase polypeptide, such as an archaeal mevalonate kinase polypeptide. The resulting isoprene compositions may have increased yields and/or purity of isoprene.
0 Citations
42 Claims
-
1-13. -13. (canceled)
-
14. A method of producing isoprene, the method comprising
(a) culturing cells comprising (i) one or more heterologous nucleic acids encoding feedback-resistant mevalonate kinase polypeptides, (ii) one or more heterologous nucleic acids encoding an isoprene synthase polypeptide or one or more additional copies of an endogenous nucleic acid encoding an isoprene synthase polypeptide, and (iii) one or more nucleic acids encoding a mevalonate (MVA) pathway polypeptide under suitable culture conditions for the production of isoprene, and (b) producing isoprene.
-
15. (canceled)
-
17. (canceled)
-
22. A method of increasing the rate or flux of production of 3,3-dimethylallyl diphosphate (DMAPP), isopentenyl diphosphate (IPP), or a product derived from 3,3-dimethylallyl diphosphate (DMAPP) or isopentenyl diphosphate (IPP) comprising:
-
(a) culturing cells comprising (i) one or more heterologous nucleic acids encoding feedback-resistant mevalonate kinase polypeptides, and (ii) one or more nucleic acids encoding a mevalonate (MVA) pathway polypeptide wherein the cells are cultured under suitable culture conditions for increasing the rate or flux of production of 3,3-dimethylallyl diphosphate (DMAPP), isopentenyl diphosphate (IPP), or a product derived from 3,3-dimethylallyl diphosphate (DMAPP) or isopentenyl diphosphate (IPP), and (b) producing 3,3-dimethylallyl diphosphate (DMAPP), isopentenyl diphosphate (IPP), or a product derived from 3,3-dimethylallyl diphosphate (DMAPP) or isopentenyl diphosphate (IPP). - View Dependent Claims (23, 24, 25)
-
-
26-28. -28. (canceled)
Specification