Test strips and preparation method thereof

• US 20130284595A1
• Filed: 04/08/2013
• Published: 10/31/2013
• Est. Priority Date: 04/09/2012
• Status: Active Grant

• Current Assignee: Compose Element Limited
• Original Assignee: Compose Element Limited
• Inventors: LIN, TSU-TAI
• Granted Patent: US 8,992,751 B2
• Time in Patent Office: Days
• US Class Current: 204/403.04
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: G01N 27/307 Disposable laminated or multilayered electrodes G01N27/3272 takes precedence
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: Y10T 156/1052 with cutting, punching, tearing or severing

Abstract

The present invention relates to test strips and preparation method thereof. More particularly, the present invention relates to a test strip comprising: (a) an insulating substrate; (b) an electrode system disposed on the insulating substrate; (c) an insulation layer disposed on the electrode system, in which an accommodation space is disposed at one side of the insulation layer for exposing a part of the electrode system to carry out an electrochemical reaction of an analyte; and (d) a gel, disposed in the accommodation space and formed by dissolving a reactive enzyme reacting with the analyte in a hydrogel and being cured.

Claims

1. A test strip comprising:

(a) an insulating substrate;

(b) an electrode system disposed on the insulating substrate;

(c) an insulation layer disposed on the electrode system, in which an accommodation space is disposed at one side of the insulation layer for exposing a part of the electrode system to carry out an electrochemical reaction of an analyte; and

(d) a gel, disposed in the accommodation space and formed by dissolving a reactive enzyme reacting with the analyte in a hydrogel and being cured.

2. The test strip of claim 1, wherein the test strip further comprises an upper cover disposed on the insulation layer.

3. The test strip of claim 1, wherein the reactive enzyme is a glucose oxidation enzyme.

4. The test strip of claim 1, wherein the analyte is a blood sugar.

5. A method of preparing a test strip comprising the steps of:

(a) providing an insulating substrate;

(b) forming an electrode system on the insulating substrate;

(c) forming an insulation layer on the electrode system, which has an accommodation space disposed at one side of the insulation layer; and

(d) providing a gel, disposed in the accommodation space and being cured after dissolving in a reactive enzyme electrochemically reacting with an analyte in a hydrogel, comprising the steps of;

(d1) providing a roller for rolling the gel;

(d2) laminating the gel on a reaction specimen and die-cutting the gel at a predetermined length; and

(d3) forming a plurality of test strips on the reaction specimen.

6. The method of claim 5, wherein the test strip further comprises an upper cover disposed on the insulation layer.

7. The method of claim 5, wherein the reactive enzyme is a glucose oxidation enzyme.

8. The method of claim 5, wherein the analyte is a blood sugar.

9. The method of claim 5, wherein the hydrogel is provided by a roller.

10. The method of claim 9, wherein the roller is a wind-type film coater device.

11. The method of claim 5, wherein the gel is cured via polymerization by UV light or piezoelectricity method or high pressure oxygen.