Methods And Apparatus For Isolating Platelets From Blood

• US 20130294983A1
• Filed: 07/01/2013
• Published: 11/07/2013
• Est. Priority Date: 05/03/2002
• Status: Active Grant

• Current Assignee: Hanuman, LLC
• Original Assignee: Hanuman, LLC
• Inventors: DORIAN, Randel, STORRS, Richard W., KING, Scott R.
• Granted Patent: US 8,950,586 B2
• Time in Patent Office: Days
• US Class Current: 422/533
• CPC Class Codes: A61K 35/00 Medicinal preparations containing materials or reaction products thereof with undetermined constitution
• CPC Class Codes: A61M 1/3693 using separation based on different densities of components, e.g. centrifuging
• CPC Class Codes: A61M 2202/0427 Platelets; Thrombocytes
• CPC Class Codes: B01D 21/0012 Settling tanks making use of filters, e.g. by floating layers of particulate material
• CPC Class Codes: B01D 21/26 Separation of sediment aided by centrifugal force or centripetal forcecentrifuges B04B; cyclones B04C
• CPC Class Codes: B01D 21/262 by using a centrifuge
• CPC Class Codes: B01D 2221/10 Separation devices for use in medical, pharmaceutical or laboratory applications, e.g. separating amalgam from dental treatment residues apparatus for dental treatment A61C17/065
• CPC Class Codes: B01L 2200/026 Fluid interfacing between devices or objects, e.g. connectors, inlet details
• CPC Class Codes: B01L 2400/0633 with moving parts
• CPC Class Codes: B01L 3/50215 using a float to separate phases
• CPC Class Codes: G01N 33/49 Blood chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Abstract

A platelet collection device comprising a centrifugal spin-separator container with a cavity having a longitudinal inner surface. A float in the cavity has a base, a platelet collection surface above the base, an outer surface. The float density is below the density of erythrocytes and above the density of plasma. The platelet collection surface has a position on the float which places it below the level of platelets when the float is suspended in separated blood. During centrifugation, a layer of platelets or buffy coat collects closely adjacent the platelet collection surface. Movement of a float having a density greater than whole blood through the sedimenting erythrocytes releases entrapped platelets, increasing the platelet yield.

Claims

1. A blood platelet collection device, comprising:

a container that partially defines a volume by a sidewall extending from a first end to a second end;

a float positioned in the volume, the float having a collection surface;

a closure at the first end of the container;

a first access port formed through the closure; and

a second access port formed through the closure;

wherein blood is introduced into the container through the first access port and wherein sedimented platelets are withdrawn through the second access port.

2. The blood platelet collection device of claim 1, wherein the second end of the container is closed.

3. The blood platelet collection device of claim 1, further comprising:

a vent for venting the container to an exterior of the container, wherein the vent, first access port, and second access port are formed through the closure.

4. The blood platelet collection device of claim 3, wherein the closure is a cap of the container.

5. The blood platelet collection device of claim 1, wherein the float further includes a base having an outer surface, where the collection surface is a platelet collection surface that is above the base and the float has a density less than a density of erythrocytes and greater than a density of plasma.

6. The blood platelet collection device of claim 1, wherein the float includes a first member and a second member separated by a platelet receptor cavity.

7. The blood platelet collection device of claim 6, wherein the first member includes the collection surface where the collection surface is concave towards the platelet receptor cavity.

8. The blood platelet collection device of claim 1, further comprising:

a plunger positioned between the float and the first end and the plunger is substantially axially concentric with the float within the volume.

9. The blood platelet collection device of claim 8, further comprising:

a projection extending from the float toward the plunger to act as a stop to limit downward movement of the plunger relative to the float.

10. The blood platelet collection device of claim 8, where the plunger further defines a central channel through the plunger configured for accessing platelet-rich fluid between a bottom of the plunger and a top of the float.

11. A blood platelet collection device, comprising:

a container having a longitudinal inner surface defining a volume;

a movable separation member positioned within the container, the separation member including a first member and a second member separated by a collection cavity, the first member defining a passage through the first member to the collection cavity and the second member defines a concave collection surface in the collection cavity; and

a port into the container, wherein fluid can be withdrawn from the collection cavity, through the passage in the first member and out of the port in the container.

12. The blood platelet collection device of claim 11, wherein the movable separation member is a float and the first member and the second member are a first member and a second member of the float coupled together to define the collection cavity therebetween.

13. The blood platelet collection device of claim 11, wherein the concave collection surface of the second member is angled towards the collection cavity.

14. The blood platelet collection device of claim 11, wherein the float has a density less than a density of erythrocytes and greater than a density of plasma, wherein the collection surface of the float is positioned at a level which passes immediately below a level of platelets after centrifugation.

15. A blood platelet collection device, comprising:

a container defining a volume within a sidewall, a first end closure, and a second end closure;

a movable separation member positioned within the container, the moveable separation member including a first member and a second member separated by third member, a collection cavity defined between the first member and the second member within the volume and the second member defines a concave collection surface in the collection cavity; and

a port through the first end closure allowing access to the volume from exterior to the container, wherein a fluid can be withdrawn from the collection cavity though the port.

16. The blood platelet collection device of claim 15, wherein the first member defines a passage through the first member to the collection cavity and the fluid can be withdrawn through the passage in the first member and out of the port in the container.

17. The blood platelet collection device of claim 16, wherein the port includes at least a first port and a second port separate from each other extending through the first end closure.

18. The blood platelet collection device of claim 15, wherein the first end closure is a cap.

19. The blood platelet collection device of claim 15, wherein the concave collection surface is tapered towards the collection cavity.

20. The blood platelet collection device of claim 15, wherein the concave collection surface is configured to mate with surface of the first member when the fluid is withdrawn from the collection cavity.