ALTERNATIVE NUCLEIC ACID SEQUENCING METHODS
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Abstract
Embodiments are provided that provide for parallel sequencing of nucleic acid segments. In some embodiments, a single sequence is sequenced by at least two different sequencing techniques and the results compared, allowing for deficiencies or strengths of one technique to be complemented by the second technique.
2 Citations
40 Claims
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1-30. -30. (canceled)
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31. A method for sequencing a polynucleotide in a preparation, said method comprising:
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obtaining a polynucleotide preparation comprising the polynucleotide, comprising amplifying the polynucleotide in a semisolid support, determining a first apparent sequence of a first region of a polynucleotide by subjecting a sample of the polynucleotide preparation to a first type of sequencing technique; determining a second apparent sequence of a second region of the polynucleotide that overlaps with the first region, by subjecting a sample of the same polynucleotide preparation to a second different type of sequencing technique; and identifying any discrepancies between the first apparent sequence and the second apparent sequence. - View Dependent Claims (32, 33, 35, 36, 37, 38, 39, 40)
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34. The method of clam 31, wherein the polynucleotide is a single molecule.
Specification