BIOEFFECTIVE KRILL OIL COMPOSITIONS

• US 20140005421A1
• Filed: 09/06/2013
• Published: 01/02/2014
• Est. Priority Date: 03/28/2007
• Status: Active Grant

• Current Assignee: Aker BioMarine Antarctic AS (Aker BioMarine ASA)
• Original Assignee: Aker BioMarine ASA
• Inventors: Bruheim, Inge, Tilseth, Snorre, Mancinelli, Daniele
• Granted Patent: US 9,375,453 B2
• Time in Patent Office: Days
• US Class Current: 554/8
• CPC Class Codes: A23L 33/115 Fatty acids or derivatives thereof; Fats or oils
• CPC Class Codes: A23V 2002/00 Food compositions, function of food ingredients or processes for food or foodstuffs
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 31/122 having the oxygen directly attached to a ring, e.g. quinones, vitamin K1, anthralin
• CPC Class Codes: A61K 31/20 having a carboxyl group bound to a chain of seven or more carbon atoms, e.g. stearic, palmitic, arachidic acids
• CPC Class Codes: A61K 31/202 having three or more double bonds, e.g. linolenic eicosanoids, e.g. leukotrienes A61K31/557
• CPC Class Codes: A61K 31/215 of carboxylic acids
• CPC Class Codes: A61K 31/23 of acids having a carboxyl group bound to a chain of seven or more carbon atoms
• CPC Class Codes: A61K 31/235 having an aromatic ring attached to a carboxyl group
• CPC Class Codes: A61K 31/575 substituted in position 17 beta by a chain of three or more carbon atoms, e.g. cholane, cholestane, ergosterol, sitosterol
• CPC Class Codes: A61K 31/683 Diesters of a phosphorus acid with two hydroxy compounds, e.g. phosphatidylinositols
• CPC Class Codes: A61K 31/685 one of the hydroxy compounds having nitrogen atoms, e.g. phosphatidylserine, lecithin
• CPC Class Codes: A61K 35/612 Crustaceans, e.g. crabs, lobsters, shrimps, krill or crayfish; Barnacles
• CPC Class Codes: A61K 45/06 Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
• CPC Class Codes: A61K 9/0053 Mouth and digestive tract, i.e. intraoral and peroral administration rectal administration A61K9/0031
• CPC Class Codes: A61K 9/48 Preparations in capsules, e.g. of gelatin, of chocolate A61K9/0004 takes precedence; bite capsules A61K9/0056
• CPC Class Codes: A61K 9/4825 Proteins, e.g. gelatin gelatin capsule shells with substantial amounts of other macromolecular substances A61K9/4816
• CPC Class Codes: A61K 9/4858 Organic compounds
• CPC Class Codes: A61P 3/02 Nutrients, e.g. vitamins, minerals
• CPC Class Codes: C11B 3/006 by extraction

Abstract

This invention discloses new krill oil compositions characterized by having high amounts of phospholipids, astaxanthin esters and/or omega-3 contents. The krill oils are obtained from krill meal using supercritical fluid extraction in a two stage process. Stage 1 removes the neutral lipid by extracting with neat supercritical CO₂ or CO₂ plus approximately 5% of a co-solvent. Stage 2 extracts the actual krill oils by using supercritical CO₂ in combination with approximately 20% ethanol. The krill oil materials obatined are compared with commercially available krill oil and found to be more bioeffective in a number of areas such as anti-inflammation, anti-oxidant effects, improving insulin resistances and improving blood lipid profile.

Claims

1. A method of production of krill oil from krill comprising:

a) denaturing the krill to provide a denatured krill product;

b) extracting the denatured krill product with supercritical fluid extraction to provide a polar krill oil comprising phospholipids;

c) formulating said polar krill oil for oral consumption.

2. The method of claim 1, wherein said supercritical fluid extraction comprises use of supercritical carbon dioxide.

3. The method of claim 1, wherein said polar krill oil comprises greater than about 40% phosphatidylcholine w/w.

4. The method of claim 1, wherein said polar krill oil comprises greater than about 45% phosphatidylcholine w/w.

5. The method of claim 1, wherein said polar krill oil comprises less than about 25% triglycerides w/w.

6. The method of claim 1, wherein said polar krill oil comprises at least 36% w/w omega-3 fatty acids.

7. The method of claim 1, wherein said krill oil comprises astaxanthin.

8. The method of claim 1, wherein said formulating further comprising formulating said polar krill oil for oral consumption by a human.

9. The method of claim 8, wherein said formulating further comprises formulating said polar krill oil in a capsule.

10. The method of claim 1, wherein said krill is Euphausia superba.

11. A method of production of krill oil from Euphausia superba krill comprising:

a) denaturing the Euphausia superba krill to provide a denatured krill product;

b) extracting the denatured krill product with supercritical fluid extraction to provide a polar krill oil comprising phospholipids;

c) formulating said polar krill oil in a capsule for oral consumption by a human.