Methods of Modifying Eukaryotic Cells
First Claim
1. An antibody having a light chain comprising a human light-chain variable region and a non-human light-chain constant region.
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Abstract
A method for engineering and utilizing large DNA vectors to target, via homologous recombination, and modify, in any desirable fashion, endogenous genes and chromosomal loci in eukaryotic cells. These large DNA targeting vectors for eukaryotic cells, termed LTVECs, are derived from fragments of cloned genomic DNA larger than those typically used by other approaches intended to perform homologous targeting in eukaryotic cells. Also provided is a rapid and convenient method of detecting eukaryotic cells in which the LTVEC has correctly targeted and modified the desired endogenous gene(s) or chromosomal locus (loci) as well as the use of these cells to generate organisms bearing the genetic modification.
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Citations
18 Claims
- 1. An antibody having a light chain comprising a human light-chain variable region and a non-human light-chain constant region.
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8. An antibody produced by a method comprising:
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a) exposing to an antigen a genetically modified mouse comprising in its germline; i. a heavy chain immunoglobulin locus comprising human unrearranged heavy chain variable region gene segments and DNA encoding a mouse heavy chain constant region; and ii. a light chain immunoglobulin locus comprising human unrearranged light chain variable region gene segments and DNA encoding a mouse heavy chain constant region; such that the genetically modified mouse produces an antibody to the antigen, wherein the antibody comprises a heavy chain comprising a human variable region and a mouse constant region, and a light chain comprising a human variable region and a mouse constant region. - View Dependent Claims (9, 10, 11, 12)
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13. An antibody produced by a method comprising:
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a) exposing to an antigen a genetically modified mouse comprising in its germline; i. a heavy chain immunoglobulin locus comprising human unrearranged heavy chain variable region gene segments and DNA encoding a mouse heavy chain constant region; and ii. a light chain immunoglobulin locus comprising human unrearranged light chain variable region gene segments and DNA encoding a mouse heavy chain constant region; b) making a hybridoma from the genetically modified mouse, the hybridoma comprising DNA encoding the heavy chain human variable region of the antibody and DNA encoding the light chain human variable region of the antibody, wherein the hybridoma produces an antibody to the antigen, wherein the antibody comprises a heavy chain comprising a human variable region and a mouse constant region, and a light chain comprising a human variable region and a mouse constant region. - View Dependent Claims (14, 15, 16, 17, 18)
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Specification