Methods and Compositions for Efficient Base Calling in Sequencing Reactions
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Abstract
The present invention is directed to methods and compositions for acquiring nucleotide sequence information of target sequences. In particular, the present invention provides methods and compositions for improving the efficiency of sequencing reactions by using fewer labels to distinguish between nucleotides and by detecting nucleotides at multiple detection positions in a target sequence.
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Citations
38 Claims
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1-21. -21. (canceled)
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22. A method for determining identities of nucleotides at detection positions of a plurality of different nucleic acid templates, said method comprising
i) performing sequencing reaction on the plurality of different templates using two distinguishable fluorescent labels; ii) determining the identities of nucleotides at detection positions based on the presence or absence of each of the two distinguishable fluorescent labels. - View Dependent Claims (23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38)
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