MAIZE PLANTS WITH IMPROVED DISEASE RESISTANCE

• US 20150026839A1
• Filed: 07/16/2014
• Published: 01/22/2015
• Est. Priority Date: 07/17/2013
• Status: Active Grant

• Current Assignee: Monsanto Technology LLC (Bayer AG)
• Original Assignee: Monsanto Technology LLC (Bayer AG)
• Inventors: Baley, George J., Jia, Hongwu, Cruz, Juan, Pan, Yule, Zhou, Hao
• Granted Patent: US 10,440,907 B2
• Time in Patent Office: Days
• US Class Current: 800/265
• CPC Class Codes: A01H 1/045 using molecular markers
• CPC Class Codes: A01H 1/1255 for fungal resistance
• CPC Class Codes: A01H 5/10 Seeds
• CPC Class Codes: A01H 6/4684 Zea mays [maize]
• CPC Class Codes: C12Q 1/6895 for plants, fungi or algae
• CPC Class Codes: C12Q 2600/13 Plant traits
• CPC Class Codes: C12Q 2600/156 Polymorphic or mutational markers

Abstract

The present invention is in the field of plant breeding and disease resistance. More specifically, the invention includes a method for breeding corn plants containing one or more markers that are associated with resistance to fungi. The invention further includes germplasm and the use of germplasm containing at least one marker associated with resistance to Fusarium stalk rot (FSR) infection for introgression into elite germplasm in a breeding program, thus producing novel FSR resistant germplasm.

Claims

1. A method of obtaining a corn plant with enhanced Fusarium stalk rot resistance comprising:

a) providing a population of corn plants;

b) detecting in said plants the presence of a Fusarium stalk rot resistance allele at a polymorphic locus genetically linked to a chromosomal segment flanked by;

marker loci psk2 and gpm753d;

or

marker loci TIDP3099 and IDP4363; and

c) selecting from said population at least a first plant comprising said allele and enhanced Fusarium stalk rot resistance compared to a plant lacking said allele.

2. The method of claim 1, wherein said segment is flanked by:

marker loci TIDP3078 and umc60;

or

marker loci TIDP3728 and TIDP5537.

3. The method of claim 1, wherein said segment is flanked by:

marker loci SEQ ID NO;

1 and SEQ ID NO;

5;

or

marker loci csu329 and IDP6942.

4. The method of claim 1, wherein said segment is flanked by:

marker loci TIDP6282 and SEQ ID NO;

4;

or

marker loci SEQ ID NO;

90 and SEQ ID NO;

2;

or

marker loci SEQ ID NO;

6 and SEQ ID NO;

17.

5. The method of claim 1, wherein said polymorphic locus comprises a nucleic acid sequence selected from the group consisting of SEQ ID NO:

1, SEQ ID NO;

2, SEQ ID NO;

3, SEQ ID NO;

4, SEQ ID NO;

5, SEQ ID NO;

6, SEQ ID NO;

7, SEQ ID NO;

8, SEQ ID NO;

9, SEQ ID NO;

10, SEQ ID NO;

11, SEQ ID NO;

12, SEQ ID NO;

13, SEQ ID NO;

14, SEQ ID NO;

15, SEQ ID NO;

16, SEQ ID NO;

17;

SEQ ID NO;

86, SEQ ID NO;

87, SEQ ID NO;

88, SEQ ID NO;

89, SEQ ID NO;

90, SEQ ID NO;

91, SEQ ID NO;

92, SEQ ID NO;

93, SEQ ID NO;

94, SEQ ID NO;

95, SEQ ID NO;

96, SEQ ID NO;

97, SEQ ID NO;

98, SEQ ID NO;

99, SEQ ID NO;

100, and SEQ ID NO;

101.

6. The method of claim 1, further defined as comprising selecting from said population at least two plants, thereby forming a population of corn plants comprising said allele and enhanced Fusarium stalk rot resistance compared to a plant lacking said allele.

7. The method of claim 1, wherein said Fusarium stalk rot resistance allele was introgressed into said population of corn plants from a starting plant or population of corn plants containing said allele.

8. The method of claim 1, further comprising producing a progeny plant with Fusarium stalk rot resistance from said first plant.

9. The method of claim 8, wherein producing the progeny plant comprises marker-assisted selection for Fusarium stalk rot resistance.

10. The method of claim 8, wherein the progeny plant is an F2-F6 progeny plant.

11. The method of claim 8, wherein producing the progeny plant comprises backcrossing.

12. A method of producing a corn plant with enhanced Fusarium stalk rot resistance comprising:

a) crossing a first corn plant comprising a Fusarium stalk rot resistance allele with a second corn plant of a different genotype to produce one or more progeny plants; and

b) selecting a progeny plant based on the presence of said allele at a polymorphic locus genetically linked to a chromosomal segment flanked by;

marker loci psk2 and gpm753d;

or

marker loci TIDP3099 and IDP4363; and

wherein said allele confers enhanced resistance to Fusarium stalk rot compared to a plant lacking said allele.

13. The method of claim 12, wherein said segment is flanked by:

marker loci TIDP3078 and umc60;

or

marker loci TIDP3728 and TIDP5537.

14. The method of claim 12, wherein said segment is flanked by:

marker loci SEQ ID NO;

1 and SEQ ID NO;

5;

or

marker loci csu329 and IDP6942.

15. The method of claim 12, wherein said segment is flanked by:

marker loci TIDP6282 and SEQ ID NO;

4;

or

marker loci SEQ ID NO;

90 and SEQ ID NO;

2;

or

marker loci SEQ ID NO;

6 and SEQ ID NO;

17.

16. The method of claim 12, wherein said polymorphic locus comprises a nucleic acid sequence selected from the group consisting of SEQ ID NO:

1, SEQ ID NO;

2, SEQ ID NO;

3, SEQ ID NO;

4, SEQ ID NO;

5, SEQ ID NO;

6, SEQ ID NO;

7, SEQ ID NO;

8, SEQ ID NO;

9, SEQ ID NO;

10, SEQ ID NO;

11, SEQ ID NO;

12, SEQ ID NO;

13, SEQ ID NO;

14, SEQ ID NO;

15, SEQ ID NO;

16, SEQ ID NO;

17;

SEQ ID NO;

86, SEQ ID NO;

87, SEQ ID NO;

88, SEQ ID NO;

89, SEQ ID NO;

90, SEQ ID NO;

91, SEQ ID NO;

92, SEQ ID NO;

93, SEQ ID NO;

94, SEQ ID NO;

95, SEQ ID NO;

96, SEQ ID NO;

97, SEQ ID NO;

98, SEQ ID NO;

99, SEQ ID NO;

100, and SEQ ID NO;

101.

17. The method of claim 12, wherein the progeny plant is an F2-F6 progeny plant.

18. The method of claim 12, wherein producing the progeny plant comprises backcrossing.

19. The method of claim 18, wherein backcrossing comprises from 2-7 generations of backcrosses.

20. The method of claim 18, wherein backcrossing comprises marker-assisted selection in at least two generations.

21. The method of claim 20, wherein backcrossing comprises marker-assisted selection in all generations.

22. The method of claim 12, wherein the first corn plant is an inbred or a hybrid.

23. The method of claim 12, wherein the second corn plant is an agronomically elite corn plant.

24. The method of claim 23, wherein the agronomically elite corn plant is an inbred or a hybrid.