ENHANCED LIGATION REACTIONS
First Claim
1. A method for ligating nucleic acids comprising:
- conducting a nucleic acid ligation reaction in the presence of an agent that catalyzes removal of an adenylate group from a terminal 5′
phosphate of a nucleic acid.
1 Assignment
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Accused Products
Abstract
In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of at least one agent that generates a ligatable terminal 5′ phosphate group by removing an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, an aprataxin enzyme can catalyze removal of an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of an aprataxin enzyme under conditions suitable for ligating nucleic acid ends.
4 Citations
20 Claims
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1. A method for ligating nucleic acids comprising:
- conducting a nucleic acid ligation reaction in the presence of an agent that catalyzes removal of an adenylate group from a terminal 5′
phosphate of a nucleic acid. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 19, 20)
- conducting a nucleic acid ligation reaction in the presence of an agent that catalyzes removal of an adenylate group from a terminal 5′
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14. A method for sequencing comprising:
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a) hybridizing a template polynucleotide to a first and second oligonucleotide probe so that the first and second oligonucleotide probes abut each other to form a nick, wherein the second oligonucleotide probe is labeled with a distinctive detectable reporter moiety; b) contacting the nick with at least one aprataxin enzyme and at least one ligase enzyme to close the nick; and c) detecting the distinctive detectable reporter moiety thereby determining the sequence of the second oligonucleotide probe hybridized to the template polynucleotide. - View Dependent Claims (15, 16, 17, 18)
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Specification