USE OF BURKHOLDERIA FORMULATIONS, COMPOSITIONS AND COMPOUNDS TO MODULATE CROP YIELD AND/OR CORN ROOTWORM INFESTATION

• US 20150079043A1
• Filed: 11/20/2014
• Published: 03/19/2015
• Est. Priority Date: 02/25/2010
• Status: Active Grant

• Current Assignee: Pro Farm Group, Inc. (Bioceres Crop Solutions Corp.)
• Original Assignee: Marrone Bio Innovations Inc. (Bioceres Crop Solutions Corp.)
• Inventors: Asolkar, Ratnakar, Koivunen, Marja, Marrone, Pamela, Cordova-Kreylos, Ana Lucia, Huang, Huazang
• Granted Patent: US 9,526,251 B2
• Time in Patent Office: Days
• US Class Current: 424/93.4
• CPC Class Codes: A01N 25/00 Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application , e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
• CPC Class Codes: A01N 25/12 Powders or granules A01N25/26 takes precedence
• CPC Class Codes: A01N 25/14 wettable
• CPC Class Codes: A01N 63/10 Animals; Substances produced thereby or obtained therefrom
• CPC Class Codes: A01N 63/20 Bacteria; Substances produced thereby or obtained therefrom
• CPC Class Codes: C07D 263/32 with only hydrogen atoms, hydrocarbon or substituted hydrocarbon radicals, directly attached to ring carbon atoms
• CPC Class Codes: C07D 263/34 with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
• CPC Class Codes: C07D 309/14 Nitrogen atoms not forming part of a nitro radical
• CPC Class Codes: C07D 407/06 linked by a carbon chain containing only aliphatic carbon atoms
• CPC Class Codes: C07D 413/04 directly linked by a ring-member-to-ring-member bond
• CPC Class Codes: C07D 413/06 linked by a carbon chain containing only aliphatic carbon atoms
• CPC Class Codes: C07D 493/10 Spiro-condensed systems
• CPC Class Codes: C07D 498/14 Ortho-condensed systems
• CPC Class Codes: C07D 513/04 Ortho-condensed systems

Abstract

Provided is the use of or compositions or formulations comprising Burkholderia species, filtrate, supernatant, extract, pesticidally active compound or metabolite derived therefrom as an insecticide, particularly against infestation of Corn Rootworm larvae, and/or as crop yield enhancer.

Claims

1. A method for modulating infestation of Diabrotica (corn rootworm) in a location where modulation is desired comprising

applying an effective amount of

(a) a culture, suspension or whole cell broth comprising a strain of Burkholderia sp., or supernatant, filtrate, cell fraction, and/or extract derived from said culture, suspension or whole cell broth, and

(b) optionally at least one of a carrier, diluent or adjuvant,

to modulate said infestation of corn rootworm at said location.

2. The method according to claim 1, wherein the location where modulation is desired is on a plant, plant seed, plant roots, plant part, seedling or substrate for growing said plant.

3. The method according to claim 1, wherein said Burkholderia sp. is Burkholderia A396 strain.

4. The method according to claim 1, wherein said Burkholderia sp. has the identifying characteristics of (NRRL Accession No. B-50319).

5. The method according to claim 1 wherein said Diabrotica (corn rootworm) is Western or Northern Corn Rootworm.

6. The method according to claim 1, wherein said Diabrotica (corn rootworm) is modulated by modulating the mortality of said corn rootworm.

7. The method according to claim 1, wherein the mortality of Diabrotica (corn rootworm) is modulated and wherein there is a mortality of corn rootworm of at least about 50% at said location.

8. The method according to claim 1, wherein the wherein the mortality of corn rootworm is modulated and wherein there is a mortality of Diabrotica (corn rootworm) of at least about 50% following application of said culture, suspension or whole cell broth comprising a strain of Burkholderia sp., or supernatant, filtrate, cell fraction, extract and/or one or more compounds derived from said culture, suspension or whole cell broth and (b) at least one of a carrier, diluent or adjuvant at said location.

9. The method according to claim 1, which further comprises applying another natural or artificial insecticidal substance.

10. The method according to claim 1, wherein the infestation of Diabrotica (corn rootworm) is modulated by modulating the rate of hatching of eggs.

11. The method according to claim 1, wherein said culture, suspension or whole cell broth comprising a strain of Burkholderia sp., or supernatant, filtrate, cell fraction, and/or extract derived from said culture, suspension or whole cell broth further comprise at least one of a carrier, diluent or adjuvant is formulated into a composition.

12. A method for modulating plant yield and Diabrotica infestation comprising applying an effective amount of a culture, suspension or whole cell broth comprising a strain of Burkholderia sp., or supernatant, filtrate, cell fraction, and/or extract derived from said culture, suspension or whole cell broth to a plant or plant part.