Sample Processing Methods

• US 20150105300A1
• Filed: 12/17/2014
• Published: 04/16/2015
• Est. Priority Date: 02/05/2003
• Status: Active Grant

• Current Assignee: Roche Molecular Systems (Roche Holding AG)
• Original Assignee: IQuum, Inc. (Roche Holding AG)
• Inventors: Chen, Shuqi, Chen, Lingjun
• Granted Patent: US 9,708,599 B2
• Time in Patent Office: Days
• US Class Current: 506/26
• CPC Class Codes: B01F 31/55 the materials to be mixed being contained in a flexible bag submitted to periodical deformation
• CPC Class Codes: B01L 2200/0621 Control of the sequence of chambers filled or emptied
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2300/042 Caps; Plugs
• CPC Class Codes: B01L 2300/046 Function or devices integrated in the closure
• CPC Class Codes: B01L 2300/047 Additional chamber, reservoir
• CPC Class Codes: B01L 2300/0681 Filter
• CPC Class Codes: B01L 2300/069 Absorbents; Gels to retain a fluid
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2300/10 Means to control humidity and/or other gases
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 2400/0677 phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers
• CPC Class Codes: B01L 3/502 with fluid transport, e.g. in multi-compartment structures centrifugal-type cuvettes G01N21/07; analysis by separation into components G01N30/00; automatic analysers G01N35/00
• CPC Class Codes: B01L 3/505 flexible containers not provided for above
• CPC Class Codes: C12N 15/1003 Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
• CPC Class Codes: G01N 1/10 in the liquid or fluent state burettes, pipettes B01L3/02; sampling of ground water E02D1/06; metering by volume of fluids or fluent solid material G01F11/00, G01F13/00
• CPC Class Codes: G01N 33/543 with an insoluble carrier for immobilising immunochemicals
• CPC Class Codes: Y02A 50/30 Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
• CPC Class Codes: Y10S 435/81 Packaged device or kit
• CPC Class Codes: Y10T 436/25 including sample preparation

Abstract

A method of processing a sample may include introducing a sample into a vessel, the vessel having proximal and distal ends, the sample being introduced into the proximal end of the vessel; incubating the sample in the vessel with a substance capable of specific binding to a preselected component of the sample; propelling components of the incubated sample, other than the preselected component, toward the proximal end of the vessel by clamping the vessel distal to the incubated sample and compressing the vessel where the incubated sample is contained; propelling the preselected component toward a distal segment of the vessel by clamping the vessel proximal to the preselected component and compressing the vessel where the preselected component is contained; and mixing the preselected component with a reagent in the distal segment of the vessel.

Claims

1. A sample processing tubule, comprising:

at least three segments, each of which is;

defined by the tubule;

fluidly isolated, at least in part by a breakable seal;

so expandable as to receive a volume of fluid expelled from another segment; and

so compressible as to contain substantially no fluid when so compressed;

wherein at least three segments each contains at least one reagent.

2. The tubule of claim 1, wherein at least a portion of the tubule is transparent.

3. The tubule of claim 1, further comprising at least one pressure gate in fluid communication with at least one segment.

4. The tubule of claim 1, further comprising at least one filter in the tubule.

5. The tubule of claim 1, wherein at least one of the reagents includes a substance capable of specific binding to a preselected component of a sample when the sample is added to the tubule.

6. The tubule of claim 5, wherein the substance is coupled to a solid substrate.

7. The tubule of claim 6, wherein the substance fauns a coating on the solid substrate.

8. The tubule of claim 6, wherein the solid substrate comprises at least one of beads, a pad, a filter, a sheet, an electrostatic surface, and a portion of a tubule wall surface.

9. The tubule of claim 6, wherein the substrate comprises at least one of silica beads, magnetic beads, silica magnetic beads, glass beads, nitrocellulose colloid beads, and magnetized nitrocellulose colloid beads.

10. The tubule of claim 6, wherein the substance comprises silica, and the substrate comprises a filter or a sheet.

11. The tubule of claim 6, wherein the substrate comprises a pad formed at least in part from an absorbent material comprising at least one of paper, film, filter, foam, mesh, and fiber matrix.

12. The tubule of claim 6, wherein the substrate is coupled to a tubule wall.

13. The tubule of claim 1, further comprising a substrate, wherein the substrate comprises a pad formed at least in part from an absorbent material comprising at least one of paper, film, filter, foam, mesh, and fiber matrix.

14. The tubule of claim 1, further comprising an open end for introducing a sample into the tubule.

15. The tubule of claim 14, further comprising a cap for closing the open end.

16. The tubule of claim 1, further comprising a frame to which the tubule is mounted.

17. The tubule of claim 1, further comprising a proximal end having an opening through which a sample is introducible, and a distal end, and wherein a second segment is distal to a first segment, and a third segment is distal to the second segment.

18. The tubule of claim 1, wherein the segments form a substantially linear array.

19. The tubule of claim 1, wherein the segments form a contiguous array.

20. A method of processing a sample, comprising:

introducing a sample into a tubule discretized by breakable seals into a plurality of fluidly isolated segments, wherein the tubule has a proximal end for receiving waste and a distal end for conducting an assay;

incubating the sample in a segment of the tubule with a substance capable of specific binding to a preselected component of the sample;

removing waste from the preselected component by clamping the tubule distally of the segment containing the preselected component and compressing that segment; and

releasing a reagent to mix with the preselected component from a fluidly isolated adjacent distal segment by compressing at least one of the segment containing the preselected component and a segment containing a reagent distal of that segment, thereby opening a breakable seal and either propelling the reagent into the segment containing the preselected component or propelling the preselected component into the segment containing the reagent.