EICOSAPENTAENOIC ACID-PRODUCING MICROORGANISMS, FATTY ACID COMPOSITIONS, AND METHODS OF MAKING AND USES THEREOF

• US 20150275244A1
• Filed: 04/28/2015
• Published: 10/01/2015
• Est. Priority Date: 07/21/2011
• Status: Active Grant

• Current Assignee: DSM IP Assets BV (DSM-Firmenich AG)
• Original Assignee: DSM IP Assets BV (DSM-Firmenich AG)
• Inventors: APT, Kirk E., BEHRENS, Paul Warren, HANSEN, Jon Milton, PFEIFER, Joseph W. III, STAHL, Tracey Lynn, ZIRKLE, Ross
• Granted Patent: US 9,611,488 B2
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: A23K 20/158 Fatty acids; Fats; Products containing oils or fats
• CPC Class Codes: A23K 50/80 for aquatic animals, e.g. fish, crustaceans or molluscs
• CPC Class Codes: A23L 2/52 Adding ingredients adding preservatives A23L2/44
• CPC Class Codes: A23L 33/12 Fatty acids or derivatives thereof
• CPC Class Codes: A23L 33/40 Complete food formulations for specific consumer groups or specific purposes, e.g. infant formula
• CPC Class Codes: A23V 2002/00 Food compositions, function of food ingredients or processes for food or foodstuffs
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 2800/10 General cosmetic use
• CPC Class Codes: A61K 31/202 having three or more double bonds, e.g. linolenic eicosanoids, e.g. leukotrienes A61K31/557
• CPC Class Codes: A61K 8/361 Carboxylic acids having more than seven carbon atoms in an unbroken chain; Salts or anhydrides thereof
• CPC Class Codes: A61P 29/00 Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
• CPC Class Codes: A61Q 19/00 Preparations for care of the skin
• CPC Class Codes: C12P 7/6427 Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone

Abstract

The invention is directed to microbial oils containing omega-3 polyunsaturated fatty acids comprising docosahexaenoic acid, eicosapentaenoic acid, and optionally docosapentaenoic acid and dosage forms containing such oils.

Claims

1-21. -21. (canceled)

22. A microbial oil comprising omega-3 polyunsaturated fatty acids comprising docosahexaenoic acid and eicosapentaenoic acid in an amount of ≧

90%, by weight, of the total amount of omega-3 polyunsaturated fatty acids, wherein the amount of eicosapentaenoic acid, by weight, is from 19% to 55% of the total amount of docosahexaenoic acid and eicosapentaenoic acid, and the amount of docosahexaenoic acid, by weight, is from 35% to 71% of the total amount of docosahexaenoic acid and eicosapentaenoic acid, and wherein the oil comprises from 120 mg to 220 mg eicosapentaenoic acid per one gram of oil and from 240 mg to 450 mg docosahexaenoic acid per one gram of oil.

26. The oil of any one of claims 22-25, wherein said oil is produced by Schizochytrium sp.

27. The oil of any one of claims 22-25, wherein said oil comprises less that about 5% by weight of each of arachidonic acid, docosapentaenoic acid n-6, oleic acid, linoleic acid, linolenic acid, eicosenoic acid, erucic acid and stearidonic acid.

29. The oil of any one of claims 22-28, wherein said oil comprises a ratio of EPA:

DHA selected from 1;

1 to 1;

1.5, 1;

1 to 1;

2, 1;

1.5 to 1;

2, 1;

1 to 1;

2.5, 1;

2 to 1;

2.5, and 1;

4 to 1;

7 by weight of total omega-3 polyunsaturated fatty acids.

23. A microbial oil comprising omega-3 polyunsaturated fatty acids comprising docosahexaenoic acid and eicosapentaenoic acid in an amount of ≧

90%, by weight, of the total amount of omega-3 polyunsaturated fatty acids, wherein the amount of eicosapentaenoic acid, by weight, is from 19% to 55% of the total amount of docosahexaenoic acid and eicosapentaenoic acid, and the amount of docosahexaenoic acid, by weight, is from 35% to 71% of the total amount of docosahexaenoic acid and eicosapentaenoic acid, and wherein the oil comprises from 130 mg to 195 mg eicosapentaenoic acid per one gram of oil and from 320 mg to 480 mg docosahexaenoic acid per one gram of oil.

24. A microbial oil comprising omega-3 polyunsaturated fatty acids comprising docosahexaenoic acid and eicosapentaenoic acid in an amount of ≧

90%, by weight, of the total amount of omega-3 polyunsaturated fatty acids, wherein the amount of eicosapentaenoic acid, by weight, is from 19% to 55% of the total amount of docosahexaenoic acid and eicosapentaenoic acid, and the amount of docosahexaenoic acid, by weight, is from 35% to 71% of the total amount of docosahexaenoic acid and eicosapentaenoic acid, and where the omega-3 polyunsaturated fatty acids further comprise docosapentaenoic acid in an amount of from 0% to 10%, by weight, of the total amount of omega-3 polyunsaturated fatty acids.

25. The oil of claim 24, wherein said oil comprises about 150 mg to about 300 mg eicosapentaenoic acid per one gram of oil;

from about 200 mg to about 400 mg docosahexaenoic acid per one gram of oil; and

from about 0 to about 55 mg docosapentaenoic acid per one gram of oil.

28. A microbial oil comprising a triacylglycerol fraction of at least 10% by weight, wherein at least about 12% by weight of the fatty acids in the triacylglycerol fraction is eicosapentaenoic acid, wherein at least 25% by weight of the fatty acids in the triacylglycerol fraction is docosahexaenoic acid, wherein less that 5% by weight of the fatty acids in the triacylglycerol fraction is arachidonic acid, and wherein the oil comprises from 120 mg to 220 mg eicosapentaenoic acid per one gram of oil and from 240 mg to 450 mg docosahexaenoic acid per one gram of oil.

30. A microbial oil comprising 120 mg to 220 mg eicosapentaenoic acid per one gram of oil and from 240 mg to 450 mg docosahexaenoic acid per one gram of oil.

31. The oil of claim 30, wherein said oil is produced by Schizochytrium sp.