PLANT-ENDOPHYTE COMBINATIONS AND USES THEREFOR
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Abstract
The disclosure provides materials and methods for conferring improved plant traits or benefits on plants. The materials can include a formulation comprising an exogenous endophytic bacterial population, which can be disposed on an exterior surface of a seed or seedling, typically in an amount effective to colonize the plant. The formulations can include at least one member selected from the group consisting of an agriculturally compatible carrier, a tackifier, a microbial stabilizer, a fungicide, an antibacterial agent, an herbicide, a nematicide, an insecticide, a plant growth regulator, a rodenticide, and a nutrient.
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35 Claims
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1-15. -15. (canceled)
- 16. A method for preparing a monocot seed comprising an endophytic bacterial population, said method comprising applying to an exterior surface of a monocot seed a formulation comprising an endophytic bacterial population consisting essentially of an endophytic bacterium comprising a 16S rRNA nucleic acid sequence at least 99% identical to a nucleic acid sequence selected from the group consisting of SEQ ID NOs:
- 28. The method of 16, wherein the seed is a corn seed, and wherein the formulation comprises at least about 1,000 CFU of the endophytic bacterium applied on the exterior surface of the seed, the formulation comprising a microbial stabilizer.
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31. A method for conferring one or more fitness benefits to a monocot agricultural plant comprising:
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a. providing a monocot seed of the plant; b. applying to an exterior surface of the monocot seed a formulation comprising an exogenous endophytic bacterial population consisting essentially of an endophytic bacterium comprising a 16S rRNA nucleic acid sequence at least 99% identical to a nucleic acid sequence selected from the group consisting of SEQ ID NOs;
1-10, wherein the exogenous endophytic bacterial population is applied to an exterior surface of the seed in an amount effective to colonize the mature plant, wherein the formulation further comprises at least one member selected from the group consisting of an agriculturally compatible carrier, a tackifier, a microbial stabilizer, a fungicide, an antibacterial agent, an herbicide, a nematicide, an insecticide, a plant growth regulator, a rodenticide, and a nutrient; andc. allowing the seed to grow under conditions that permit the endophytic bacterium to colonize the plant. - View Dependent Claims (32, 33, 34)
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35. A method of obtaining greater flowering uniformity in a population of agricultural monocot plants comprising:
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a. providing a population of monocot seeds; b. applying to an exterior surfaces of the population of seeds a formulation comprising an exogenous endophytic bacterial population consisting essentially of an endophytic bacterium comprising a 16S rRNA nucleic acid sequence at least 99% identical to a nucleic acid sequence selected from the group consisting of SEQ ID NOs;
1-10, wherein the exogenous endophytic bacterial population is applied on an exterior surface of the seed in an amount effective to colonize the mature plant, wherein the formulation further comprises at least one member selected from the group consisting of an agriculturally compatible carrier, a tackifier, a microbial stabilizer, a fungicide, an antibacterial agent, an herbicide, a nematicide, an insecticide, a plant growth regulator, a rodenticide, and a nutrient; andc. allowing the population of seeds to grow at least to the flowering stage, wherein the population of plants has a reduction in the standard deviation in the flowering time of at least 5% when compared with a population of reference agricultural plants grown under the same conditions.
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Specification