NOVEL POLYMERS AND DNA COPOLYMER COATINGS

• US 20160122816A1
• Filed: 10/29/2015
• Published: 05/05/2016
• Est. Priority Date: 10/31/2014
• Status: Active Grant

• Current Assignee: Illumina Cambridge Limited (Illumina Incorporated)
• Original Assignee: Illumina Cambridge Limited (Illumina Incorporated)
• Inventors: Brown, Andrew A., George, Wayne N., Richez, Alexandre, Dingwall, Anne-Cecile, von Hatten, Xavier
• Granted Patent: US 9,815,916 B2
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: C08F 20/60 containing nitrogen in addition to the carbonamido nitrogen
• CPC Class Codes: C08F 220/22 Esters containing halogen
• CPC Class Codes: C08F 220/282 and containing two or more oxygen atoms
• CPC Class Codes: C08F 220/325 containing glycidyl radical, e.g. glycidyl (meth)acrylate
• CPC Class Codes: C08F 220/346 and further oxygen
• CPC Class Codes: C08F 220/56 Acrylamide; Methacrylamide
• CPC Class Codes: C08F 220/60 containing nitrogen in addition to the carbonamido nitrogen
• CPC Class Codes: C08F 220/603 and containing oxygen in addition to the carbonamido oxygen and nitrogen
• CPC Class Codes: C08F 222/385 Monomers containing two or more (meth)acrylamide groups, e.g. N,N'-methylenebisacrylamide
• CPC Class Codes: C08F 2438/03 Use of a di- or tri-thiocarbonylthio compound, e.g. di- or tri-thioester, di- or tri-thiocarbamate, or a xanthate as chain transfer agent, e.g . Reversible Addition Fragmentation chain Transfer [RAFT] or Macromolecular Design via Interchange of Xanthates [MADIX]
• CPC Class Codes: C08F 285/00 Macromolecular compounds obtained by polymerising monomers on to preformed graft polymers
• CPC Class Codes: C08F 293/005 using free radical "living" or "controlled" polymerisation, e.g. using a complexing agent
• CPC Class Codes: C08F 8/32 by reaction with amines
• CPC Class Codes: C12Q 1/6874 involving nucleic acid arrays, e.g. sequencing by hybridisation
• CPC Class Codes: C12Q 2565/401 Signal amplification by chemical polymerisation
• CPC Class Codes: C40B 50/18 using a particular method of attachment to the solid support

Abstract

Some embodiments described herein relate to new polymer coatings for surface functionalization and new processes for grafting pre-grafted DNA-copolymers to surface(s) of substrates for use in DNA sequencing and other diagnostic applications.

Claims

1. A polymer for surface functionalization, comprising a recurring unit of Formula (I) and a recurring unit of Formula (II):

2. The polymer of claim 1, wherein R^1a is selected from hydrogen or optionally substituted alkyl and each of R^2a and R^3a is hydrogen.

3. The polymer of claim 1, wherein R^1b is selected from hydrogen or optionally substituted alkyl and each of R^2b and R^3b is hydrogen.

4. The polymer of claim 1, wherein L² is an optionally substituted alkylene.

5. The polymer of claim 1, wherein L¹ is an optionally substituted alkylene.

6. The polymer of claim 1, wherein the recurring unit of Formula (I) and Formula (II) are also represented by Formula (Ia) and Formula (IIa):

7. The polymer of claim 1, wherein L¹ is an optionally substituted heteroalkylene.

8. The polymer of claim 7, wherein L¹ is —

(CH₂)m-NH—

(CH₂)n- optionally substituted with one or more oxo, and wherein each m and n is an integer independently selected from 1 to 10.

9. The polymer of claim 1, wherein the recurring unit of Formula (I) is also represented by Formula (Ib):

10. The polymer of claim 1, further comprises one or more recurring units selected from the group consisting of polyacrylamides, polyacrylates, polyurethanes, polysiloxanes, silicones, polyacroleins, polyphosphazenes, polyisocyanates, poly-ols, and polysaccharides, or combinations thereof.

11. The polymer of claim 10, further comprises one or more recurring units of Formula (IIIa) or (IIIb) or both:

12. The polymer of claim 11, wherein each R^4a, R^4b and R^5b is selected from hydrogen or methyl.

13. The polymer of claim 11, wherein R^6b and R^7b are both hydrogen.

14. The polymer of claim 11, wherein at least one of R^5a or R^6a is hydrogen.

15. The polymer of claim 11, wherein at least one of R^5a or R^6a is methyl.

16. The polymer of claim 11, wherein the recurring units of Formula (IIIa) is also represented by (IIIa1), (IIIa2) or (IIIa3):

17. The polymer of claim 11, wherein the recurring units of Formula (IIIb) is also represented by (IIIb1):

18. A substrate having a first surface comprising a polymer of claim 1 covalently bonded thereto.

19. A polymer for surface functionalization, comprising a recurring unit of Formula (IV):

20. A polymer for surface functionalization, comprising a recurring unit of Formula (V):

21. A method for immobilizing a grafted polymer to a first surface of a substrate, comprising:

providing a substrate having a first surface comprising a first plurality of functional groups covalently attached thereto;

providing a grafted polymer comprising functionalized oligonucleotides covalently bonded to a polymer, wherein the polymer comprises a second plurality of functional groups; and

reacting the first plurality functional groups of the first surface with the second plurality of functional groups of the polymer such that the polymer is covalently bonded to the first surface of the substrate;

provided that when the first plurality of functional groups comprise norbornene, the functionalized oligonucleotides comprise bicyclo[6.1.0]non-4-yne;

then the polymer is not a polymer of the following structures;