MULTI-WELL FOURIER PTYCHOGRAPHIC AND FLUORESCENCE IMAGING
First Claim
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16. A system for Fourier ptychographic imaging and fluorescence imaging, the system comprising:
- a body configured to receive a multi-well plate; and
imaging devices arranged for one to one correspondence with wells in the multi-well plate, each imaging device comprising;
a first fluorescence illumination source configured to provide excitation light of a first range of wavelengths to the corresponding well;
an optical system with a pair of objectives positioned oppositely; and
an image sensor for capturing intensity measurements based on light received from the corresponding well,wherein the system generates an improved resolution brightfield image of the sample using Fourier ptychographic reconstruction based on a sequence of uniquely illumination intensity measurements acquired during sequential illumination at different illumination angles by a variable illumination source; and
wherein the system generates a first fluorescence image based on light emitted by the sample in response to receiving excitation light of the first range of wavelengths.
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Abstract
Certain aspects pertain to multi-well systems, devices, and methods of Fourier ptychographic and fluorescence imaging.
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Citations
33 Claims
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16. A system for Fourier ptychographic imaging and fluorescence imaging, the system comprising:
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a body configured to receive a multi-well plate; and imaging devices arranged for one to one correspondence with wells in the multi-well plate, each imaging device comprising; a first fluorescence illumination source configured to provide excitation light of a first range of wavelengths to the corresponding well; an optical system with a pair of objectives positioned oppositely; and an image sensor for capturing intensity measurements based on light received from the corresponding well, wherein the system generates an improved resolution brightfield image of the sample using Fourier ptychographic reconstruction based on a sequence of uniquely illumination intensity measurements acquired during sequential illumination at different illumination angles by a variable illumination source; and wherein the system generates a first fluorescence image based on light emitted by the sample in response to receiving excitation light of the first range of wavelengths. - View Dependent Claims (1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29)
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25-1. The system of claim 16, wherein each objective has a numerical aperture of less than 0.55.
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30. An imaging method, comprising:
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sequentially illuminating a multi-well plate with plane wave illumination at a plurality of illumination angles using a variable illumination source; for each well in the well plate, acquiring a sequence of uniquely illuminated intensity measurements from light passing through a sample in the corresponding well based on sequential illumination at the plurality of illumination angles; and for each well in the well plate, reconstructing with a Fourier ptychography reconstruction process an improved resolution brightfield image of the sample based on the sequence of uniquely illuminated intensity measurements. - View Dependent Claims (31, 32, 33)
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Specification