MASSIVELY PARALLEL SINGLE CELL ANALYSIS
First Claim
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1. A composition comprisinga support;
- anda plurality of oligonucleotides associated with the support, wherein each of the plurality of oligonucleotides comprises a cellular label sequence and a molecular label sequence, wherein the cellular label sequence of each of the plurality of oligonucleotides is the same, and at least 100 of the plurality of oligonucleotides comprise different molecular label sequences.
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Abstract
The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).
50 Citations
20 Claims
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1. A composition comprising
a support; - and
a plurality of oligonucleotides associated with the support, wherein each of the plurality of oligonucleotides comprises a cellular label sequence and a molecular label sequence, wherein the cellular label sequence of each of the plurality of oligonucleotides is the same, and at least 100 of the plurality of oligonucleotides comprise different molecular label sequences. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
- and
- 16. A kit comprising a plurality of supports and a plurality of oligonucleotides associated with each of the plurality of supports, wherein each of the plurality of oligonucleotides comprises a cellular label sequence and a molecular label sequence, wherein the cellular label sequence of each of the plurality of oligonucleotides is the same, and at least 100 of the plurality of oligonucleotides comprise different molecular label sequences.
Specification