COMPOSITIONS FOR LINKING DNA-BINDING DOMAINS AND CLEAVAGE DOMAINS
First Claim
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1. A nuclease comprising:
- a DNA-binding domain that binds to a nucleotide target site; and
a FokI cleavage domain, wherein the FokI cleavage domain comprises a substitution of amino acid residues 161 and 162 of SEQ ID NO;
1 with an amino acid sequence EAAARE (SEQ ID NO;
5) or an insertion after amino acid residue 162 of SEQ ID NO;
1 of an amino acid sequence KSEAAAR (SEQ ID NO;
6).
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Abstract
Disclosed herein are compositions for linking DNA binding domains and cleavage domains (or cleavage half-domains) to form non-naturally occurring nucleases. Also described are methods of making and using compositions comprising these linkers.
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14 Claims
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1. A nuclease comprising:
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a DNA-binding domain that binds to a nucleotide target site; and a FokI cleavage domain, wherein the FokI cleavage domain comprises a substitution of amino acid residues 161 and 162 of SEQ ID NO;
1 with an amino acid sequence EAAARE (SEQ ID NO;
5) or an insertion after amino acid residue 162 of SEQ ID NO;
1 of an amino acid sequence KSEAAAR (SEQ ID NO;
6). - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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Specification