Non-Contact Monitoring of Biofilms and Corrosion on Submerged Surfaces with Electrochemical Impedance Spectroscopy

• US 20160334350A1
• Filed: 05/12/2015
• Published: 11/17/2016
• Est. Priority Date: 05/12/2015
• Status: Active Grant

• Current Assignee: Battelle Savannah River Alliance LLC
• Original Assignee: Savannah River Nuclear Solutions LLS
• Inventors: Turick, Charles E., Milliken, Charles E., Colon-Mercado, Hector, Greenway, Scott D.
• Granted Patent: US 10,234,376 B2
• Time in Patent Office: Days
• US Class Current: 1/1
• CPC Class Codes: C12Q 1/02 involving viable microorganisms
• CPC Class Codes: G01N 17/02 Electrochemical measuring systems for weathering, corrosion or corrosion-protection measurement
• CPC Class Codes: G01N 17/04 Corrosion probes
• CPC Class Codes: G01N 33/4833 of solid biological material, e.g. tissue samples, cell cultures tissue in vivo A61B5/00; cell suspensions G01N33/48735

Abstract

Systems and methods for monitoring microorganisms on a surface are provided. In particular, a flat, patterned sensing electrode can be positioned proximate a surface in non-contact relationship with the surface. The sensing electrode can include a working electrode and a counter electrode. The surface and the sensing electrode can be submerged in an aqueous medium. An alternating current signal can be applied at the working electrode. The signal can propagate through the aqueous medium and can be measured at the counter electrode. The presence of microorganisms on the surface can cause changes in the signal as the signal propagates through the aqueous medium. Such changes in the signal can be used to determine impedance parameters, which can correspond to microbial characteristics associated with the surface. For instance, the microbial characteristics can be associated with a biofilm, corrosion and/or bio-corrosion on the surface.

Claims

1. A system for monitoring microorganisms on a surface, the system comprising:

a flat, patterned electrode having a working electrode and a counter electrode, the flat, patterned electrode positioned proximate the surface in non-contact relationship with the surface;

a signal source coupled to the flat, patterned electrode, the signal source configured to apply an alternating current signal to the working electrode at one or more frequencies; and

a processing device configured to detect a measured signal at the counter electrode;

wherein one or more characteristics of the measured signal at the counter electrode are indicative of one or more characteristics of microorganisms on the surface.

2. The system of claim 1, wherein the surface is submerged in an aqueous medium.

3. The system of claim 2, wherein the sensing electrode is positioned in the aqueous medium a range of about 0.1 millimeters to about 100 centimeters above the surface.

4. The system of claim 1 wherein the one or more characteristics of microorganisms on the surface are associated with at least one of a biofilm on the surface, corrosion on the surface, or bio-corrosion on the surface.

5. The system of claim 1, wherein the measured signal at the counter electrode comprises a current signal.

6. The system of claim 1, wherein the one or more characteristics of the measured signal comprise a phase shift of the measured signal relative to the applied signal.

7. The system of claim 6, wherein the phase shift is indicative of at least one of geochemical change and microbial density.

8. The system of claim 6, wherein the one or more characteristics of the measured signal comprise one or more impedance parameters associated with a biofilm on the surface, the one or more impedance parameters determined based at least in part from the phase shift.

9. The system of claim 8, wherein the one or more impedance parameters comprise at least one of an absolute impedance, real impedance or imaginary impedance associated with the biofilm.

10. The system of claim 8, wherein at least one of the one or more impedance parameters is indicative of microbial activity associated with the surface.

11. The system of claim 1, the flat, patterned electrode further having a reference electrode.

12. A method of monitoring microorganisms on a surface, the method comprising:

positioning a flat, patterned electrode proximate a surface in non-contact relationship with the surface, the flat patterned electrode comprising a counter electrode and a working electrode;

applying an alternating current signal at the working electrode;

detecting a measured signal at the counter electrode; and

determining one or more characteristics of the measured signal;

wherein the one or more characteristics of the measured signal are indicative of one or more characteristics of microorganisms on the surface.

13. The method of claim 12, wherein the one or more characteristics of the measured signal comprise impedance parameters associated with the surface.

14. The method of claim 13, wherein the impedance parameters comprise at least one of an absolute impedance, real impedance or imaginary impedance associated with the surface.

15. The method of claim 12, wherein the one or more characteristics of microorganisms on the surface are associated with at least one of a biofilm on the surface, corrosion on the surface, or bio-corrosion on the surface.

16. The method of claim 12, wherein the surface and the flat, patterned electrode are submerged in an aqueous medium, and the flat, patterned electrode is positioned 3 millimeters above the surface.

17. A system for monitoring one or more characteristics of a biofilm on a surface;

the system comprising;

a flat, patterned electrode having a working electrode and a counter electrode, the flat, patterned electrode positioned proximate the surface in non-contact relationship with the surface, wherein the flat, patterned electrode and the surface are submerged in an aqueous medium;

a signal source coupled to the flat, patterned electrode, the signal source configured to apply an alternating current potential at the working electrode at one or more frequencies; and

one or more processing devices configured to detect a measured alternating current at the counter electrode, the one or more processing devices further configured to determine one or more impedance parameters associated with the surface based at least in part on the applied potential and the measured current;

wherein the one or more impedance parameters are indicative of microbial activity associated with the biofilm.

18. The system of claim 17, wherein the one or more impedance parameters are determined based at least in part on a phase shift of the measured current relative to the applied potential.

19. The system of claim 18, wherein the phase shift is indicative of one or more characteristics of the biofilm on the surface.

20. The system of claim 17, wherein the microbial activity associated with the biofilm comprises microbial growth.