SUBPOPULATIONS OF SPORE-LIKE CELLS AND USES THEREOF
First Claim
1. A composition comprising a sub-population of spore-like cells expressing one or more markets selected from the group consisting of Oct4, nanog, Zfp296, crypto, Gdf3, UtF1, Ecat1, Esg1, Sox2, Pax6, SCA-1, CD29, CD34, CD90, B1 integrin, cKit, SP-C, CC10, SF1, DAX1, and SCG10, wherein the cells remain viable following oxygen deprivation for at least four hours before isolation in a physiologically acceptable carrier.
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Accused Products
Abstract
Subpopulations of spore-like cells expressing specific cell surface and gene expression markers are provided. In one embodiment, the cells express at least one cell surface or gene expression marker selected from the group consisting of Oct4, nanog, Zfp296, cripto, Gdf3, UtF1, Ecat1, Esg1, Sox2, Pax6, nestin, SCA-1, CD29, CD34, CD90, B1 integrin, cKit, SP-C, CC10, SF1, DAX1, and SCG10. Also provided are methods for purifying a subpopulation of spore-like cells of interest from a population of spore-like cells, and methods for inducing differentiation of the isolated spore-like cells into cells of endodermal, mesodermal or ectodermal origin. The spore-like cells can be used to generate cells originating from all three germ layers and can be used to treat a patient who has a deficiency of functional cells in any of a wide variety of tissues, including the retina, intestine, bladder, kidney, liver, lung, nervous system, or endocrine system.
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Citations
10 Claims
- 1. A composition comprising a sub-population of spore-like cells expressing one or more markets selected from the group consisting of Oct4, nanog, Zfp296, crypto, Gdf3, UtF1, Ecat1, Esg1, Sox2, Pax6, SCA-1, CD29, CD34, CD90, B1 integrin, cKit, SP-C, CC10, SF1, DAX1, and SCG10, wherein the cells remain viable following oxygen deprivation for at least four hours before isolation in a physiologically acceptable carrier.
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10. The method of claim 16, wherein the damage tissue is selected from the group consisting of a burn, a bone fracture, a torn ligament, an atrophied muscle, an ischemic heart or a malfunctioning gland.
Specification