LOW LIGNIN NON-TRANSGENIC ALFALFA VARIETIES AND METHODS FOR PRODUCING THE SAME
First Claim
1. A Medicago sativa seed designated as CW 054004, wherein a sample of said seed has been deposited as ATCC Accession Number PTA-122470.
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Abstract
Disclosed are alfalfa varieties with low or reduced lignin content, including an alfalfa seed designated CW 096043 deposited as ATCC Accession Number PTA-122473, an alfalfa seed designated CW 103009 deposited as ATCC Accession Number PTA-122475, an alfalfa seed designated CW 099079 deposited as ATCC Accession Number PTA-122474, an alfalfa seed designated CW 090075 deposited as ATCC Accession Number PTA-122471, an alfalfa seed designated CW 054004 deposited as ATCC Accession Number PTA-122470, an alfalfa seed designated CW 093009 deposited as ATCC Accession Number PTA-122472, and an alfalfa seed designated CW 104015 deposited as ATCC Accession Number PTA-122476. Also disclosed are plants, or parts thereof, grown from the seed of the cultivar, plants having the morphological and physiological characteristics of these alfalfa varieties, such as CW 096043, CW 103009, CW 099079, CW 090075, CW 054004, CW 093009, or CW 104015 and methods of using the plant or parts thereof in alfalfa breeding and alfalfa transformation.
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20 Claims
- 1. A Medicago sativa seed designated as CW 054004, wherein a sample of said seed has been deposited as ATCC Accession Number PTA-122470.
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14. A method for producing an alfalfa cultivar CW 054004-derived alfalfa plant, comprising:
- (a) crossing CW 054004 plants grown from CW 054004 seed, representative seed of which has been deposited under ATCC Accession No;
PTA-122470, with a second alfalfa plant to yield progeny alfalfa seed; and
(b) growing the progeny seed to yield an alfalfa cultivar CW 054004-derived alfalfa plant. - View Dependent Claims (15, 16)
- (a) crossing CW 054004 plants grown from CW 054004 seed, representative seed of which has been deposited under ATCC Accession No;
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17. A method of introducing a desired trait into alfalfa CW 054004 comprising:
- (a) crossing CW 054004 plants grown from CW 054004 seed, representative seed of which has been deposited under ATCC Accession No;
PTA-122475, with plants of a second alfalfa variety that comprise a desired trait to produce F1 progeny plants, wherein the desired trait is selected from the group consisting of male sterility, site-specific recombination, increased transformability, abiotic stress tolerance, herbicide resistance, insect resistance, disease resistance, altered phosphorus, altered antioxidants, altered fatty acids, altered essential amino acids and altered carbohydrates;
(b) selecting F1 progeny plants that have the desired trait to produce selected F1 progeny plants;
(c) crossing the selected progeny plants with the CW 054004 plants to produce backcross progeny plants;
(d) selecting for backcross progeny plants that have the desired trait and physiological and morphological characteristics of alfalfa variety CW 054004 to produce selected backcross progeny plants; and
(e) repeating steps (c) and (d) three or more times in succession to produce selected fourth or higher backcross progeny plants that comprise the desired trait and all the physiological and morphological characteristics of alfalfa variety CW 054004. - View Dependent Claims (18)
- (a) crossing CW 054004 plants grown from CW 054004 seed, representative seed of which has been deposited under ATCC Accession No;
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19. A method for producing an alfalfa plant having an altered agronomic trait comprising introducing a polynucleotide into a CW 054004 plant grown from CW 054004 seed, representative seed of which has been deposited under ATCC Accession No:
- PTA-122475, wherein the polynucleotide confers an altered agronomic trait to the plant selected from the group consisting of insect resistance, disease resistance, herbicide resistance, abiotic stress resistance, fatty acid metabolism, starch metabolism, carbohydrate metabolism, amino acid metabolism, phosphorus metabolism, antioxidant metabolism, male sterility, site specific DNA integration, plant growth, forage yield and flowering, and wherein the polynucleotide is expressed in the plant.
- View Dependent Claims (20)
Specification