HYDROPHILIC COATING OF FLUIDIC CHANNELS
First Claim
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1. A device comprising:
- a flowcell comprising a fluidic channel, an inlet port, and an outlet port,wherein the fluidic channel comprises a ceiling, a first sidewall, and a fluidic channel bottom, wherein the ceiling and the first sidewall form a ceiling-first sidewall edge, and wherein the bottom and the first sidewall for a bottom-first sidewall edge,wherein the contact angle of the ceiling is at least 10 degrees smaller than the contact angle of the first sidewall,wherein the bottom of the fluidic channel comprises a substrate which comprises a plurality of microwells, andwherein the inlet port and the outlet port are in fluid communication with the flowcell via the fluidic channel.
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Abstract
Disclosed herein are methods, devices, and systems for efficient loading and retrieval of particles. In some embodiments, a fluidic channel of a flowcell comprises a ceiling, a first sidewall, and a bottom, wherein the contact angle of the ceiling is at least 10 degrees smaller than the contact angle of the first sidewall, and wherein the bottom of the fluidic channel comprises a substrate that comprises a plurality of microwells.
7 Citations
30 Claims
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1. A device comprising:
a flowcell comprising a fluidic channel, an inlet port, and an outlet port, wherein the fluidic channel comprises a ceiling, a first sidewall, and a fluidic channel bottom, wherein the ceiling and the first sidewall form a ceiling-first sidewall edge, and wherein the bottom and the first sidewall for a bottom-first sidewall edge, wherein the contact angle of the ceiling is at least 10 degrees smaller than the contact angle of the first sidewall, wherein the bottom of the fluidic channel comprises a substrate which comprises a plurality of microwells, and wherein the inlet port and the outlet port are in fluid communication with the flowcell via the fluidic channel. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27)
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28. A method for sample loading, comprising
(a) providing a device that comprises: -
a flowcell comprising a fluidic channel, an inlet port, and an outlet port, wherein the fluidic channel comprises a ceiling, a first sidewall, and a bottom, wherein the ceiling and the first sidewall forms a ceiling-first sidewall edge, and wherein the bottom and the first sidewall forms a bottom-first sidewall edge, wherein the contact angle of the ceiling is at least 10 degrees smaller than the contact angle of the first sidewall, wherein the bottom comprises a substrate which comprises a plurality of microwells, and wherein the inlet port and the outlet port are in fluid communication with the flowcell via the fluidic channel; (b) introducing a gas into the fluidic channel via the inlet port; and (c) introducing a first sample comprising a first plurality of particles into the fluidic channel via the inlet port, whereby at least 25% of the plurality of microwells each contains a single particle of the first plurality of particles. - View Dependent Claims (29, 30)
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Specification