PROCESS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL AND FERMENTATION OF SUGARS

• US 20180208949A1
• Filed: 03/23/2018
• Published: 07/26/2018
• Est. Priority Date: 11/09/2012
• Status: Active Grant

• Current Assignee: Versalis S.P.A, (Eni Petroleum Company Incorporated)
• Original Assignee: DSM IP Assets BV (DSM-Firmenich AG)
• Inventors: NOORDAM, Bertus, BERKHOUT, Michael Petrus Jozef, HISENI, Aida
• Granted Patent: US 10,724,057 B2
• Time in Patent Office: Days
• CPC Class Codes: C12P 17/10 Nitrogen as only ring hetero atom
• CPC Class Codes: C12P 19/02 Monosaccharides 2-ketogulonic acid C12P7/60
• CPC Class Codes: C12P 19/14 produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase , e.g. by cellulase, hemicellulase
• CPC Class Codes: C12P 2203/00 Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source ethanol C12P7/10
• CPC Class Codes: C12P 35/00 Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
• CPC Class Codes: C12P 5/026 Unsaturated compounds, i.e. alkenes, alkynes or allenes
• CPC Class Codes: C12P 7/10 substrate containing cellulosic material
• CPC Class Codes: C12P 7/16 Butanols
• CPC Class Codes: C12P 7/18 polyhydric
• CPC Class Codes: C12P 7/20 Glycerol
• CPC Class Codes: C12P 7/40 containing a carboxyl group including Peroxycarboxylic acids
• CPC Class Codes: C12P 7/42 Hydroxy-carboxylic acids
• CPC Class Codes: C12P 7/46 Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
• CPC Class Codes: C12P 7/48 Tricarboxylic acids, e.g. citric acid
• CPC Class Codes: C12P 7/54 Acetic acid vinegar C12J
• CPC Class Codes: C12P 7/56 Lactic acid
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel
• CPC Class Codes: Y02E 50/30 Fuel from waste, e.g. synthetic alcohol or diesel

Abstract

The invention relates to a process for the preparation of a sugar product from ligno-cellulosic material, comprising the following steps:

• • a) optionally pre-treatment of the ligno-cellulosic material;
  • b) optionally washing of the optionally pre-treated ligno-cellulosic material;
  • c) enzymatic hydrolysis of the optionally washed and/or optionally pre-treated ligno-cellulosic material using an enzyme composition comprising at least two cellulase and whereby the enzyme composition at least comprises GH61; and
  • d) optionally recovery of a sugar product;
    wherein during part of the time of the enzymatic hydrolysis, oxygen is added to the ligno-cellulosic material and during part of the time of the enzymatic hydrolysis less oxygen is added to the ligno-cellulosic material compared to the other part of the time of the enzymatic hydrolysis, preferably no oxygen is added to the ligno-cellulosic material.

Claims

1. A process for the preparation of a sugar product from ligno-cellulosic material, comprising the following:

a) optional pre-treatment of the ligno-cellulosic material;

b) optional washing of the optionally pre-treated ligno-cellulosic material;

c) enzymatic hydrolysis of the optionally washed and/or optionally pre-treated ligno-cellulosic material using an enzyme composition comprising at least two cellulases and whereby the enzyme composition at least comprises GH61; and

d) optional recovery of a sugar product;

wherein during part of the time of the enzymatic hydrolysis, oxygen is added to the ligno-cellulosic material and during part of the time of the enzymatic hydrolysis less oxygen is added to the ligno-cellulosic material compared to the other part of the time of the enzymatic hydrolysis, preferably no oxygen is added to the ligno-cellulosic material.

3. The process according to claim 1, wherein the part of the time wherein less or optionally no oxygen is added is 10 to 80%, optionally 20 to 80%, optionally 30 to 80% and optionally 40 to 80% of the total enzymatic hydrolysis time.

4. The process according to claim 1, wherein the part of the time wherein more oxygen is added is 2 to 80%, optionally 4 to 60%, optionally 8 to 50% and optionally 10 to 50% of the total enzymatic hydrolysis time.

5. The process according to claim 4, wherein the part of the time wherein more oxygen is added is

a) 12 to 50%, and optionally 20 to 40% when the oxygen is added in the second half of time of the enzymatic hydrolysis;

b) 2 to 30%, optionally 4 to 25% and optionally 5 to 20% of the total enzymatic hydrolysis time when the oxygen is added in the first half of time of the enzymatic hydrolysis;

or

c) or a combination of a and b.

6. The process according to claim 1 whereby the oxygen concentration in the liquid phase of the hydrolysis during the part of the time wherein oxygen is added is at least 2 times, optionally at least 4 times, optionally at least 10 times the oxygen concentration in the liquid phase during the part of the time wherein less or no oxygen is added.

7. The process according to claim 1 wherein in the part of the time when the oxygen is added, the oxygen concentration in the liquid phase, wherein the ligno-cellulosic material is present during the enzymatic hydrolysis, is at least 0.001 mol/m³, optionally at least 0.002 mol/m³ and optionally at least 0.003 mol/m³ or wherein in the part of the time when the oxygen is added, the oxygen concentration in the liquid phase, wherein the ligno-cellulosic material is present during the enzymatic hydrolysis, is at most 0.12, optionally at most 0.09 mol/m³, optionally at most 0.06 mol/m³, optionally at most 0.045 mol/m³ and optionally at most 0.03 mol/m³.

8. The process according to claim 1, wherein the oxygen is added in the form of bubbles.

9. The process according to claim 1, wherein the reactor for the enzymatic hydrolysis has a volume of 1 m³ or more.

10. The process according to claim 1, wherein the enzymatic hydrolysis time is 5 to 150 hours.

11. The process according to claim 1, wherein the enzyme composition used retains activity for 30 hours or more.

12. The process according to claim 1, wherein the hydrolysis is conducted at a temperature of 45°

C. or more, optionally at a temperature of 50°

C. or more and optionally at a temperature of 55°

C. or more.

13. The process according to claim 1, wherein the enzyme composition is from a fungus, optionally a microorganism of genus Rasamsonia or the enzyme composition comprises a fungal enzyme optionally a Rasamsonia enzyme.

14. The process according to claim 1, wherein the dry matter content in the hydrolysis step c) is 10 wt % or more, optionally 14 wt % or more and optionally from 14 to 33% wt %.

15. The process according to claim 1 in which the enzymatic hydrolysis takes place in a batch, fed batch and/or continuous culture reactor.

16. The process according to claim 1 in which oxygen is introduced as an oxygen-containing gas, optionally air.

2. A process for the preparation of a fermentation product from ligno-cellulosic material, comprising the following:

a) optional pre-treatment of the ligno-cellulosic material;

b) optional washing of the optionally pre-treated ligno-cellulosic material;

c) enzymatic hydrolysis of the optionally washed and/or optionally pre-treated ligno-cellulosic material using an enzyme composition comprising at least two cellulases and whereby the enzyme composition at least comprises GH61;

d) fermentation of the hydrolysed ligno-cellulosic material to produce a fermentation product; and

e) optional recovery of a fermentation product;

wherein during part of the time of the enzymatic hydrolysis, oxygen is added to the ligno-cellulosic material and during part of the time of the enzymatic hydrolysis less oxygen is added to the ligno-cellulosic material compared to the other part of time of the enzymatic hydrolysis, preferably no oxygen is added to the ligno-cellulosic material.