Methods for Producing Canola Plants with Clubroot Resistance and Compositions Thereof

• US 20180305774A1
• Filed: 04/27/2016
• Published: 10/25/2018
• Est. Priority Date: 04/30/2015
• Status: Active Grant

• Current Assignee: Monsanto Technology LLC (Bayer AG)
• Original Assignee: Monsanto Technology LLC (Bayer AG)
• Inventors: CHRISTIANSON, Jed A., COULIBALY, Issa, NINO-LIU, David O., WU, Chunren X.
• Granted Patent: US 10,415,101 B2
• Time in Patent Office: Days
• CPC Class Codes: A01H 1/045 using molecular markers
• CPC Class Codes: A01H 5/10 Seeds
• CPC Class Codes: A01H 6/202 Brassica napus [canola]
• CPC Class Codes: C12Q 1/6895 for plants, fungi or algae
• CPC Class Codes: C12Q 2600/13 Plant traits
• CPC Class Codes: C12Q 2600/156 Polymorphic or mutational markers
• CPC Class Codes: C12Q 2600/172 Haplotypes

Abstract

The present disclosure is in the field of plant breeding and disease resistance. The disclosure provides methods for breeding canola plants having clubroot resistance using marker-assisted selection. The disclosure further provides germplasm resistant to various Plasmodiophora brassicae pathotypes including pathogtype 5x. The disclosure also provides markers associated with clubroot resistance loci for introgressing these loci into elite germplasm in a breeding program, thus producing novel clubroot resistant germplasm, e.g., spring canola varieties resistant to pathogtype 5x.

Claims

1. A method for producing a clubroot-resistant canola plant, said method comprising:

a. detecting in a population of canola plants a canola plant comprising a clubroot resistant allele at a polymorphic locus within 10 cM of any one of marker loci SEQ ID NOs;

1 to 8; and

b. selecting said canola plant comprising said clubroot resistant allele.

2. The method of claim 1, wherein said polymorphic locus is within about 5 cM of any one of marker loci SEQ ID NOs:

1 to 8.

3. The method of claim 1, wherein said polymorphic locus is located in a chromosomal segment flanked by any two of marker loci SEQ ID NOs:

9 to 12.

4. The method of claim 1, wherein said step (a) comprises genotyping said population of canola plants via a marker detection assay to determine said clubroot resistant allele.

5. The method of claim 1, wherein said step (a) comprises detecting a haplotype.

6. The method of claim 4, wherein said haplotype comprises resistance alleles of any two of marker loci SEQ ID NOs:

1 to 8.

7. The method of claim 1, wherein said selected canola plant is a Brassica napus plant or a Brassica rapa plant.

8. The method of claim 1, wherein said selected canola plant is from a spring canola variety.

9. The method of claim 1, wherein said selected canola plant is highly resistant, resistant or moderately resistant to at least one P. brassicae pathotype selected from the group consisting of pathotypes 2, 3, 5, 5x, 6, 8, and G.

10. The method of claim 1, wherein said method further comprises crossing a first canola plant comprising said clubroot resistant allele with a second canola plant to produce said population of canola plants.

11. The method of claim 1, further comprising producing a population of progenies from said selected canola plant.

12. A method for creating a population of canola plants with clubroot resistance, said method comprising:

a. genotyping a first population of canola plants with a marker closely linked to a first clubroot resistance locus and with a marker closely linked to a second clubroot resistance locus, wherein said first clubroot resistance locus is located in a chromosomal segment flanked by any two of marker loci SEQ ID NOs;

1 to 8;

b. selecting from said first population one or more canola plants containing said first and second clubroot resistance loci; and

c. producing a population of offspring from said selected one or more canola plants.

13. The method of claim 12, wherein said second clubroot resistance locus is selected from the group consisting of Crr1, Crr2, Crr3, Crr4, CRa, CRb, CRc, and CRk.

14. The method of claim 12, wherein said second clubroot resistance locus is located in a chromosomal segment flanked by any two of marker loci SEQ ID NOs:

9 to 12.

15. A spring canola variety comprising clubroot resistance conferred by a clubroot resistance locus in a chromosomal segment flanked by any two of marker loci SEQ ID NOs:

1 to 8.

16. The spring canola variety of claim 15, wherein said variety is highly resistant to, resistant to, or moderately resistant to at least one, at least two, or at least three P. brassicae pathotypes selected from the group consisting of pathotypes 1 to 9, G, H, and 5x.

17. The spring canola variety of claim 15, wherein said variety is highly resistant to, resistant to, or moderately resistant to at least one, at least two, or at least three P. brassicae pathotypes selected from the group consisting of 2, 3, 5, 5x, 6, 8, and G.

18. The spring canola variety of claim 15, wherein said variety is homozygous at said clubroot resistance locus.

19. The spring canola variety of claim 15, wherein said variety is an elite canola variety.

20. The spring canola variety of claim 15, wherein a clubroot resistance allele of said clubroot resistance locus is not present in Tosca.