SAMPLE ANALYZER AND COMPUTER PROGRAM PRODUCT

• US 20190107533A1
• Filed: 12/10/2018
• Published: 04/11/2019
• Est. Priority Date: 02/01/2007
• Status: Active Grant

• Current Assignee: Sysmex America, Inc. (Sysmex Corporation), Sysmex Corporation
• Original Assignee: Sysmex Corporation
• Inventors: NAGAI, Takaaki, NARISADA, Noriyuki, FUKUMA, Daigo, IMAZU, Masanori
• Granted Patent: US 10,401,350 B2
• Time in Patent Office: Days
• CPC Class Codes: G01N 15/12 by observing changes in resistance or impedance across apertures when traversed by individual particles, e.g. by using the Coulter principle
• CPC Class Codes: G01N 15/1459 the analysis being performed on a sample stream
• CPC Class Codes: G01N 2015/012 Red blood cells
• CPC Class Codes: G01N 2015/014 Reticulocytes
• CPC Class Codes: G01N 2015/016 White blood cells
• CPC Class Codes: G01N 2015/1486 Counting the particles
• CPC Class Codes: G01N 33/5091 for testing the pathological state of an organism
• CPC Class Codes: G01N 33/5094 for blood cell populations red blood cells G01N33/80
• CPC Class Codes: G01N 33/721 Haemoglobin
• CPC Class Codes: G01N 33/726 Devices
• CPC Class Codes: Y10T 436/11 Automated chemical analysis
• CPC Class Codes: Y10T 436/113332 with conveyance of sample along a test line in a container or rack

Abstract

A sample analyzer prepares a measurement sample from a blood sample or a body fluid sample which differs from the blood sample; measures the prepared measurement sample; obtains characteristic information representing characteristics of the components in the measurement sample; sets either a blood measurement mode for measuring the blood sample, or a body fluid measurement mode for measuring the body fluid sample as an operating mode; and measures the measurement sample prepared from the blood sample by executing operations in the blood measurement mode when the blood measurement mode has been set, and measuring the measurement sample prepared from the body fluid sample by executing operations in the body fluid measurement mode that differs from the operations in the blood measurement mode when the body fluid measurement mode has been set, is disclosed. A computer program product is also disclosed.

Claims

1. A sample analyzer comprising:

a plurality of detectors each configured to optically or electrically sense cells in a sample for measurement of the cells in the sample, the sample selectively comprising (i) a blood sample or (ii) a body fluid sample, wherein the body fluid sample contains body fluid, other than blood, which comprises one of cerebrospinal fluid, thoracic fluid, abdominal fluid, fluid collected in the cardiac sac, synovial fluid, dialysate from peritoneal dialysis, or intraperitoneal rinse;

a controller programmed to selectively operate the sample analyzer in a blood measuring mode or a body fluid measuring mode, wherein the blood measuring mode includes a sequence of operations for measuring cells in the blood sample, and the body fluid measuring mode includes a sequence of operations for measuring cells in the body fluid sample, and wherein the plurality of detectors includes one or more multi-mode detectors configured to operate in both the blood measuring mode and the body fluid measuring mode, the controller programmed to;

perform the sequence of operations in the blood measuring mode to;

introduce the blood sample into a multi-mode detector;

operate said multi-mode detector to sense cells in the introduced blood sample; and

derive measurements of individual cells in the introduced blood sample; and

perform the sequence of operations in the body fluid measuring mode to;

introduce the body fluid sample into said multi-detector;

operate said multi-mode detector to sense cells in the introduced body fluid sample; and

derive measurements of individual cells in the introduced body fluid sample.

2. The sample analyzer according to claim 1, wherein

the sequence of operations performed in the blood measuring mode comprises an operation of sensing the cells in the introduced blood sample for a first measurement time, using said multi-mode detector, and

the sequence of operation performed in the body fluid measuring mode comprises an operation of sensing the cells in the introduced body fluid sample for a second measurement time, using said multi-mode detector, wherein the second measurement time is longer than the first measurement time according to a cell concentration of the sample.

3. The sample analyzer according to claim 1, wherein the sequence of operations performed in the body fluid measuring mode comprises an operation of pre-washing said multi-mode detector to reduce a carryover effect on measurements of the cells in the body fluid sample, wherein said pre-washing is programmed to occur before introducing the body fluid sample into said multi-mode detector during said sequence of operations.

4. The sample analyzer according to claim 3, wherein an operation of pre-washing said multi-mode detector performed before introducing the body fluid sample into said multi-mode detector is not included in the sequence of operations performed in the blood measuring mode.

5. The sample analyzer according to claim 3, wherein the operation of pre-washing includes using solution different from solution for washing said multi-mode detector in the blood measuring mode.

6. The sample analyzer according to claim 1, wherein in both the blood measuring mode and the body fluid measuring mode, the sample is mixed with one or more reagent before being sensed for measurement.

7. The sample analyzer according to claim 1, wherein the sequence of operations performed in the body fluid measuring mode comprises operations of analyzing the measurements of the cells in the introduced body fluid sample, and counting a type of cells among the cells in the introduced body fluid sample.

8. The sample analyzer according to claim 6, wherein the sequence of operations performed in the body fluid measuring mode comprises an operation of counting at least one of mono-nucleated cells or poly-nucleated cells among the cells in the introduced body fluid sample.

9. The sample analyzer according to claim 5, wherein the sequence of operations performed in the body fluid measuring mode comprises an operation of calculating a relative amount of the at least one of mono-nucleated cells or poly-nucleated cells.

10. The sample analyzer according to claim 5, wherein the sequence of operations performed in the blood measuring mode comprises an operation of counting white blood cells.

11. The sample analyzer according to claim 1, wherein the controller is programmed to:

introduce a cell-free sample into said multi-mode detector, the cell-free sample having no cells contained in the cell-free sample; and

sense the cell-free sample by said multi-mode detector, and

the controller is further programmed to analyze the measurements of the cell-free sample and count cells carried over into the cell-free sample from a test sample previously measured.

12. A sample analyzer comprising:

a plurality of detectors each configured to optically or electrically sense cells in a sample for measurement of the cells in the sample, the sample selectively comprising (i) a blood sample or (ii) a body fluid sample, wherein the body fluid sample contains body fluid, other than blood, which comprises one of cerebrospinal fluid, thoracic fluid, abdominal fluid, fluid collected in the cardiac sac, synovial fluid, dialysate from peritoneal dialysis, or intraperitoneal rinse;

a controller programmed to selectively operate the sample analyzer in a blood measuring mode or a body fluid measuring mode, wherein the blood measuring mode includes a sequence of operations for measuring cells in the blood sample, and the body fluid measuring mode includes a sequence of operations for measuring cells in the body fluid sample, and wherein the plurality of detectors includes one or more multi-mode detectors configured to operate in both the blood measuring mode and the body fluid measuring mode, the controller programmed to;

perform the sequence of operations in the blood measuring mode to;

introduce the blood sample into a multi-mode detector;

operate said multi-mode detector to sense cells in the introduced blood sample; and

derive measurements of individual cells in the introduced blood sample, wherein the sequence of operations performed in the blood measuring mode comprises operations of analyzing the measurements of the individual cells in the introduced blood sample, and counting a particular type of cells in the introduced blood sample; and

perform the sequence of operations in the body fluid measuring mode to;

introduce the body fluid sample into said multi-mode detector;

operate said multi-mode detector to sense cells in the introduced body fluid sample; and

derive measurements of individual cells in the introduced body fluid sample, wherein the sequence of operations performed in the body fluid measuring mode comprises operations of analyzing the measurements of the individual cells in the introduced body fluid sample, and counting the particular type of cells in the introduced body fluid sample.

13. The sample analyzer according to claim 12, wherein the particular type of cells are blood cells.

14. The sample analyzer according to claim 12, wherein the particular type of cells are white blood cells or a subclass of the white blood cells.

15. The sample analyzer according to claim 12, wherein the particular type of cells are red blood cells.

16. The sample analyzer according to claim 12, wherein the particular type of cells are platelets.

17. The sample analyzers according to claim 12, wherein the controller is programmed to operate said multi-mode detector in the blood measuring mode for a first measurement time to sense the particular type of cells in the introduced blood sample, and

the controller is programmed to operate said multi-mode detector in the body fluid measuring mode for a second measuring time to sense the particular type of cells in the introduced body fluid sample, wherein the second measurement time is longer than the first measurement time according to a cell concentration of the sample.

18. The sample analyzers according to claim 12, wherein the sequence of operations performed in the body fluid measuring mode comprises an operation of pre-washing said multi-mode detector to reduce a carryover effect on measurements of the body fluid sample, wherein said pre-washing is programmed to occur before introducing the body fluid sample into said multi-mode detector during said sequence of operations.

19. A sample analyzer comprising:

a plurality of detectors each configured to optically or electrically sense cells in a sample for measurement of the cells in the sample, the sample selectively comprising (i) a blood sample or (ii) a body fluid sample, wherein the body fluid sample contains body fluid, other than blood, which comprises one of cerebrospinal fluid, thoracic fluid, abdominal fluid, fluid collected in the cardiac sac, synovial fluid, dialysate from peritoneal dialysis, or intraperitoneal rinse;

a controller programmed to selectively operate the sample analyzer in a blood measuring mode or a body fluid measuring mode, wherein the blood measuring mode includes a sequence of operations for measuring cells in the blood sample, and the body fluid measuring mode includes a sequence of operations for measuring cells in the body fluid sample, and wherein the plurality of detectors includes one or more multi-mode detectors configured to operate in both the blood measuring mode and the body fluid measuring mode, the controller programmed to;

perform the sequence of operations in the blood measuring mode to;

introduce the blood sample into a multi-mode detector;

operate said multi-mode detector to sense cells in the introduced blood sample; and

derive measurements of individual cells in the introduced blood sample; and

perform the sequence of operations in the body fluid measuring mode to;

introduce the body fluid sample into said multi-mode detector;

operate said multi-mode detector to sense cells in the introduced body fluid sample; and

derive measurements of individual cells in the introduced body fluid sample,

wherein the sequence of operations performed in the body fluid measuring mode comprises an operation of pre-washing said multi-mode detector to reduce a carryover effect on measurements of the body fluid sample, wherein said pre-washing is programmed to occur before introducing the body fluid sample into said multi-mode detector during said sequence of operations.

20. A sample analyzer comprising:

a plurality of detectors each configured to optically or electrically sense cells in a sample for measurement of the cells in the sample, the sample selectively comprising (i) a blood sample or (ii) a body fluid sample, wherein the body fluid sample contains body fluid, other than blood, which comprises one of cerebrospinal fluid, thoracic fluid, abdominal fluid, fluid collected in the cardiac sac, synovial fluid, dialysate from peritoneal dialysis, or intraperitoneal rinse;

a controller programmed to selectively operate the sample analyzer in a blood measuring mode or a body fluid measuring mode, wherein the blood measuring mode includes a sequence of operations for measuring cells in the blood sample, and the body fluid measuring mode includes a sequence of operations for measuring cells in the body fluid sample, the controller programmed to;

perform the sequence of operations in the blood measuring mode to;

introduce the blood sample into one of the plurality detectors;

operate said one of the plurality of detectors to optically sense cells in the introduced blood sample; and

derive measurements of individual cells in the introduced blood sample, wherein the sequence of operations performed in the blood measuring mode comprises operations of analyzing the measurements of the individual cells in the introduced blood sample, and counting each of five types of white blood cells in the introduced blood sample; and

perform the sequence of operations in the body fluid measuring mode to;

introduce the body fluid sample into and operate another of the plurality of detectors to electrically sense individual cells in the introduced body fluid sample; and

derive measurements of individual cells in the introduced body fluid sample, wherein the sequence of operations performed in the body fluid measuring mode comprises operations of analyzing the measurements of the individual cells in the introduced body fluid sample, and counting each of mono-nucleated cells and poly-nucleated cells in the introduced body fluid sample.