METHOD AND AN APPARATUS FOR DOSING REAGENTS AND FOR THEIR INCUBATION AND FOR SAMPLING REACTION MIXTURE
First Claim
2. The method of claim 1, wherein said samples are removed from said reaction syringes at the same time that said stopping liquid is passed synchronously from said syringes containing said stopping liquid, said samples and said stopping liquid being passed to the same point.
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Abstract
A method and an apparatus for dosing reagents and for incubation and sampling of the reaction mixture, in which method the reagents are drawn into the reaction syringes in which the incubation is carried out and in which the samples are discharged from the syringes in one or more portions, the apparatus consisting of a syringe unit, reaction syringes mounted on a single frame which is easy to replace, syringes containing stopping liquid and an internal standard and mounted on a fixed outer frame, vessels for the reagents, instruments for feeding reagents and for the automatic adjustment of the pistons and for transferring absorption plates, and a thermostat. The invention is suitable for reactions and dosing purposes, particularly for macromolecular investigation of radioactive parent substances, where the sample is absorbed into a porous medium and where it is washed automatically.
15 Citations
13 Claims
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2. The method of claim 1, wherein said samples are removed from said reaction syringes at the same time that said stopping liquid is passed synchronously from said syringes containing said stopping liquid, said samples and said stopping liquid being passed to the same point.
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3. The method of claim 1, wherein said sample and said stopping liquid are absorbed into an absorption plate.
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4. The method of claim 1, wherein said sample is absorbed into an absorption plate containing said stopping liquid absorbed therein.
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5. The method of claim 1, wherein said mixing of said reagent in said reaction syringes is carried out magnetically.
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6. The method of claim 1, wherein said reaction syringes are replaceable single-use syringes and said reagent vessels are changeable boat-like vessels.
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7. An apparatus for dosing reagents and for incubation and sampling of a reaction mixture obtained during incubation of said reagents which comprises a reaction syringe unit containing a desired amount of reaction syringes with pistons, syringes for containing a stopping solution for stopping the reaction in said reaction mixture, vessels for said reagents and a tank for said stopping liquid, means for feeding said reagents from said reagent vessels into said reaction syringes and means for feeding said stopping liquid from the tank containing it into said stopping liquid syringes, means for automatically adjusting the movement of said pistons of said reaction syringes and said stopping liquid syringes, means for discharging the contents of said syringes into absorption plates, means for thermostating said reaction syringes during incubation, and a control unit for controlling said apparatus.
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8. The apparatus of claim 7, wherein said reaction syringes are mounted on a single frame, said frame being changeable as a unit.
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9. The apparatus as claimed in claim 7, including a conveyor for positioning said absorption plates under said syringe unit and removing them therefrom.
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10. The apparatus as claimed in claim 7, including magnets in said reaction syringe for mixing said reaction mixture, said magnets being moved by a solenoid by means of an iron core fixed into said permanent magnet.
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11. The appratus as claimed in claim 7, wherein said pistons of said reaction syringes and said stopping liquid syringes have a coupled moving means comprising a stepmotor driven automatically by a control means of said control unit, which moves said moving means the needed distance, the pistons of said syringes being fixed.
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12. The apparatUs as claimed in claim 7, wherein said absorption plates are separated by intermediate plates and situated in their own absorption compartments which together form a movable absorption vessel.
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13. The apparatus as claimed in claim 12, wherein said absorption plate is formed of compact surface layers and loose material therebetween, said material being a gel when macromolecular synthesis is investigated.
Specification