Method of analyzing liquid samples and system for continuously automatically analyzing same

• US 4,160,646 A
• Filed: 11/21/1977
• Issued: 07/10/1979
• Est. Priority Date: 01/20/1977
• Status: Expired due to Term

• Current Assignee: Kabushiki Kaisha Kyoto Daiichi Kagaku
• Original Assignee: Kabushiki Kaisha Kyoto Daiichi Kagaku
• Inventors: Furutani, Yoshikazu, Kishimoto, Shinichi
• Primary Examiner(s): Serwin, R. E.
• Application Number:
  US05/853,137
• Time in Patent Office: 596 Days
• Field of Search: 23/253 R, 23/253 TP, 23/230 R, 356/212, 256/199, 256/209
• US Class Current: 436/169
• CPC Class Codes: G01N 2035/00039 Transport arrangements specific to flat sample substrates, e.g. pusher blade
• CPC Class Codes: G01N 2035/00118 for multiple tests
• CPC Class Codes: G01N 21/8483 Investigating reagent band test-element handling not specific to a test method G01N33/4875; analytical elements specific to chemical analysis of biological material G01N33/52; autometer with reagent band G01N35/04
• CPC Class Codes: G01N 2201/065 Integrating spheres
• CPC Class Codes: G01N 35/00029 provided with flat sample substrates, e.g. slides G01N35/028 takes precedence
• CPC Class Codes: Y10T 436/110833 Utilizing a moving indicator strip or tape

Abstract

A method of analyzing a liquid sample by applying the sample to a composite test paper composed of a strip plastic transparent film and a reflectivity reference or compensation paper piece and a plurality of color reaction test paper pieces affixed to the strip, feeding the test paper to a spectral reflectivity measuring device a predetermined period of time after the application, and calculating the corrected reflectivities of each of the paper pieces from the measured amounts of reflected light at varying wavelengths to determine the concentration of the abnormal substance contained in the test sample, free of measuring errors due to the color if any of the sample, differences in the color developed and variations in the thickness of the color reaction test paper pieces and reflectivity reference or compensation paper piece. A system for practicing this method is also disclosed.

Claims

1. A method of analyzing a liquid sample comprising the steps of applying the sample to a composite test medium formed of a strip having thereon a reflectivity reference piece and at least one color reaction test piece feeding the test medium to spectral reflectivity measuring means, irradiating each of the pieces with energy of a plurality of predetermined different wavelengths, and obtaining corrected reflectivities of each of the test pieces as the relative values with respect to the reflectivity of said reflectivity reference piece from the amounts of reflected energy at at least some of the different wavelengths to measure the concentration of an abnormal substance contained in the test sample.

2. A method of analyzing a liquid sample as in claim 1 wherein the corrected reflectivities are obtained as the relative values of the reflectivities of a test piece at the different wavelengths based on the reflectivities, calculated as 100%, of the reference piece at the corresponding wavelengths, and the concentration of the abnormal substance is measured by the corrected reflectivities at selected wavelengths of the different wavelengths.

3. A method of analyzing a liquid sample as in claim 1 wherein among the relative values of the reflectivities of a test piece at the different wavelengths based on the reflectivities, calculated as 100%, of the reference piece at the corresponding wavelengths, the relative value at one of the wavelengths giving a large variation in the amount of reflected energy is divided by the relative value at another wavelength giving a small variation in the amount of reflected energy, the corrected reflectivity being expressed in terms of the resulting quotient.

8. A method as in claim 2 wherein the concentration of the abnormal substance is measured by the corrected reflectivities of all of the different wavelengths.

4. A system for continuously automatically analyzing liquid samples comprising a sample holder for successively receiving sheets of composite test media in a first position, means for intermittently moving said sample holder, reflectivity measuring means, wavelength selector means disposed adjacent the sample holder for directing energy of several predetermined and different wavelengths through said reflectivity measuring means to irradiate the test media with the energy of selected wavelengths, scanning means disposed adjacent the sample holder and operative while the holder is stopped for moving a test media relative to said selector means, detector means for receiving the output of said reflectivity measuring means, energy transmitted through said test media and energy produced by said wavelength selector means, and a data processing means operative in response to signals from said detector means for determining the reflectivity of the test media.

5. A system for continuously automatically analyzing liquid samples as in claim 4 wherein the wavelength selector means comprises a light source, filter folder means, means for rotating said filter holder means at a constant speed, and a plurality of filters held by said filter holder means and equidistantly spaced apart for passing light rays of predetermined wavelengths, the filters being positionable concentrically with the optical axis of the light, and wavelength identifying signal generator means for such wavelength selector means.

6. A system for continuously automatically analyzing a liquid sample as in claim 5 wherein the wavelength identifying signal generator means comprises apertures formed in the filter holder means and positioned outward of the filters fitted in the holder means, said light source means and light detectors positionable respectively above and below the apertures when the center of the filters is in alignment with the optical axis of the light source means.

7. A system for continuously automatically analyzing a liquid sample as in claim 4 wherein the reflectivity measuring means comprises an integrating sphere and the light detector means therefore is mounted on the integrating sphere.

9. A method of analyzing a liquid sample comprising the steps of:

applying the sample to at least one color reaction test piece,

irradiating the test piece with sample applied thereto to a plurality of predetermined different wavelengths of energy,

and measuring the spectral reflectance of the energy reflected from the test piece at each of said predetermined different wavelengths.

10. A method as in claim 9 further comprising the steps of:

providing a reflective reference piece,

irradiating said reflective reference piece with said plurality of different wavelengths of energy and measuring the spectral reflectance therefrom at each of said predetermined different wavelengths, and

obtaining a corrected spectral reflectance for the test piece from the measurements of the spectral reflectance of both the test piece and the reflective reference piece.

11. A method as in claim 9 wherein the irradiation step is carried out by sequentially irradiating the test piece with the different wavelengths of energy.

12. A method as in claim 10 wherein the irradiation step is carried out by sequentially irradiating the test piece and the reflective reference piece with the different wavelengths of energy.

13. A system for analyzing for different substances in a liquid sample which has been applied to a test piece comprising:

means for irradiating the test piece with light energy of a plurality of different, predetermined wavelengths,

means for measuring the reflectance of the light from the test piece of at least a selected plurality of the different wavelengths, and

means responsive to the measured values of reflected light for analyzing for the substance.

14. A system as in claim 13 wherein said test piece is located on a carrier, said carrier further including a reference piece of a given reflectivity, said irradiating means irradiating both said test piece and said reference piece with light energy of a plurality of different predetermined wavelengths, said measuring means being responsive to the light reflected from both said test piece and said reference piece for producing corrected relative values of the amount of light reflected at a plurality of the selected wavelengths.

15. A system as in claim 14 wherein said carrier for the pieces comprises a strip and said irradiating means comprises a fixed position light source, and means for moving the strip relative to the light source.

16. A system as in claim 15 wherein said irradiating means also includes a plurality of filter means each defining one of said plurality of wavelengths, and means for moving said filter means relative to said light source.