Means for separating lymphocytes and monocytes from anticoagulated blood
First Claim
1. A method for separating platelets, lymphocytes, and monocytes from anticoagulant blood comprising the steps:
- (a) a water-insoluble thixotropic gel-like substance which is chemically inert to blood constituents and having a specific gravity between 1.065-1.077 g/cc is placed in a sample of anticoagulated blood;
(b) the gel-blood sample is centrifuged at a force of at least 1200 G'"'"'s for a sufficient length of time to cause the gel-like substance to form a barrier between the plasma, platelets, lymphocytes, and monocytes and the heavier blood cells; and
, thereafter,(c) the plasma, platelets, lymphocytes, and monocytes are removed from atop said barrier.
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Accused Products
Abstract
This invention relates to a method for isolating platelets, lymphocytes, and monocytes from anticoagulated blood. The method involves centrifuging at high force a sample of uncoagulated blood with a water-insoluble thixotropic gel-like material having a specific gravity between 1.065-1.077 g/cc for a length of time sufficient to cause the gel-like material to form a barrier between the platelets, lymphocytes, monocytes, and plasma fraction of the blood and the heavier blood cells. Thereafter, the plasma is withdrawn and the platelets, lymphocytes, and monocytes removed from above the barrier in a buffer solution. Where desired, the platelets can be separated from the lymphocytes and monocytes utilizing a similar process but wherein the water-insoluble thixotropic gel-like material has a specific gravity of less than about 1.055 g/cc.
168 Citations
29 Claims
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1. A method for separating platelets, lymphocytes, and monocytes from anticoagulant blood comprising the steps:
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(a) a water-insoluble thixotropic gel-like substance which is chemically inert to blood constituents and having a specific gravity between 1.065-1.077 g/cc is placed in a sample of anticoagulated blood; (b) the gel-blood sample is centrifuged at a force of at least 1200 G'"'"'s for a sufficient length of time to cause the gel-like substance to form a barrier between the plasma, platelets, lymphocytes, and monocytes and the heavier blood cells; and
, thereafter,(c) the plasma, platelets, lymphocytes, and monocytes are removed from atop said barrier. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. A method for separating platelets, lymphocytes, and monocytes from anticoagulant blood comprising the steps:
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(a) a water-insoluble thixotropic gel-like substance which is chemically inert to blood constituents and having a specific gravity between 1.065-1.077 g/cc is placed in a sample of anticoagulated blood; (b) the gel-blood sample is centrifuged at a force of at least 1200 G'"'"'s for a sufficient length of time to cause the gel-like substance to form a barrier between the plasma, platelets, lymphocytes and monocytes and the heavier blood cells; (c) the plasma is removed from atop said barrier leaving said platelets, lymphocytes and monocytes resting thereupon; (d) a buffer solution compatible with platelets, lymphocytes, and monocytes, inert to the gel-like substance, and having a specific gravity less than that of the gel-like substance is deposited upon said barrier; (e) the buffer solution is agitated to cause the said platelets, lymphocytes, and monocytes to become suspended therein; and
then(f) the suspension of platelets, lymphocytes, and monocytes in buffer is removed from contact with said barrier. - View Dependent Claims (9, 10, 11, 12, 13, 14, 15)
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16. A method for separating platelets from lymphocytes and monocytes comprising the steps:
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(a) a water-insoluble thixotropic gel-like substance which is chemically inert to blood constituents and having a specific gravity between 1.03-1.055 g/cc is placed in a blood plasma sample of anticoagulated blood containing platelets, lymphocytes, and monocytes; (b) the gel-plasma sample is centrifuged at a force of at least 1200 G'"'"'s for a sufficient length of time to cause the gel-like substance to form a barrier between the plasma and platelets and the lymphocytes and monocytes; and
, thereafter,(c) the plasma and platelets are removed from atop said barrier. - View Dependent Claims (17, 18, 19, 20, 21, 22)
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23. A method for separating platelets from anticoagulant blood comprising the steps:
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(a) a water-insoluble thixotropic gel-like substance which is chemically inert to blood constituents and having a specific gravity between 1.03-1.055 g/cc is placed in a sample of anticoagulated blood; (b) the gel-blood sample is centrifuged at a force of at least 1200 G'"'"'s for a sufficient length of time to cause the gel-like substance to form a barrier between the plasma and platelets and the heavier blood cells; and
, thereafter,(c) the plasma and platelets are removed from atop said barrier. - View Dependent Claims (24, 25, 26, 27, 28, 29)
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Specification