Means for separating lymphocytes and monocytes from anticoagulated blood

  • US 4,190,535 A
  • Filed: 07/10/1978
  • Issued: 02/26/1980
  • Est. Priority Date: 02/27/1978
  • Status: Expired due to Term
First Claim
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1. A method for separating platelets, lymphocytes, and monocytes from anticoagulant blood comprising the steps:

  • (a) a water-insoluble thixotropic gel-like substance which is chemically inert to blood constituents and having a specific gravity between 1.065-1.077 g/cc is placed in a sample of anticoagulated blood;

    (b) the gel-blood sample is centrifuged at a force of at least 1200 G'"'"'s for a sufficient length of time to cause the gel-like substance to form a barrier between the plasma, platelets, lymphocytes, and monocytes and the heavier blood cells; and

    , thereafter,(c) the plasma, platelets, lymphocytes, and monocytes are removed from atop said barrier.

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