Method and apparatus for performing in vitro clinical diagnostic tests using a solid phase assay system

• US 4,225,575 A
• Filed: 05/15/1978
• Issued: 09/30/1980
• Est. Priority Date: 05/15/1978
• Status: Expired due to Term

• Current Assignee: Ventrex Laboratories, Inc.
• Original Assignee: Ventrex Laboratories, Inc.
• Inventors: Piasio, Roger N., Perry, David A., Nayak, Pangal N.
• Primary Examiner(s): Padgett, Benjamin R.
• Assistant Examiner(s): Nucker, Christine M.
• Application Number:
  US05/905,552
• Time in Patent Office: 869 Days
• Field of Search: 424/1, 424/12, 206/206, 206/569, 23/253, 23/259, 23/230 B, 195/103.5 A, 422/57, 422/58
• US Class Current: 436/518
• CPC Class Codes: C12Q 1/00 Measuring or testing processes involving enzymes, nucleic acids or microorganisms measuring or testing apparatus with condition measuring or sensing means, e.g. colony counters, C12M1/34; Compositions therefor; Processes of preparing such compositions
• CPC Class Codes: G01N 33/50 Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing measuring or testing processes involving enzymes or microorganisms, compositions or test papers therefor; processes for forming such compositions, condition responsive control in microbiological or enzymological processes C12Q
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing
• CPC Class Codes: Y10S 436/801 Electron dense compounds, e.g. ferritin
• CPC Class Codes: Y10S 436/804 Radioisotope, e.g. radioimmunoassay
• CPC Class Codes: Y10S 436/81 Tube, bottle, or dipstick

Abstract

A method and apparatus for conducting chemical reactions at a liquid-solid interface wherein a reaction component is fixed on the surface of a solid phase immersed in a liquid phase containing a freely diffusing, mobile reaction component. The apparatus comprises a substantially smooth-surfaced matrix of appropriate geometrical configuration designed to provide a large surface to volume ratio, a short transfer distance from the mobile component to the fixed component distributed on the solid phase surface and to drain freely upon removal from the reaction liquid.

Claims

1. A process for determining the amount of mobile component contained in a fluid sample which reacts with a fixed component immobilized on a solid phase insert matrix to produce a measurable change which is a function of the amount of the mobile component comprising the steps of:

(a) providing a fluid receptacle containing a fluid sample having an unknown amount of the mobile component which is to be measured,

(b) providing a removable impermeable unitary insert matrix comprising a handle member having attached at one end a plurality of elements having essentially smooth plane or curved surfaces so arranged with respect to one another and the handle member and of a size and shape such that when said matrix is inserted into said fluid receptacle, said matrix extends substantially throughout the depth of said fluid sample and the average diffusion distance of molecules of said mobile component to the surfaces of said elements is greatly reduced relative to the average diffusion distance of molecules of said mobile component to the fluid receptacle when no matrix is present therein, and having the fixed component immobilized on the surfaces of said elements, capable of reacting with said mobile component at a rate or to an extent measurable as a function of the concentration of said mobile component,

(c) placing the insert in the fluid receptacle to cause contact between the fixed component of the insert matrix with the mobile component of unknown amount contained in the fluid sample for a given time interval to permit a reaction to occur, and

(d) measuring the change which is a function of the concentration of the mobile component to determine the amount of the mobile component.

2. The process of claim 1 wherein the fixed component is an antibody, the mobile component is an antigen and the reaction is the binding of the antigen to the antibody.

3. The process of claim 2 wherein at least a portion of the antigen contains a radioactive label and the amount of radioactivity bound to the antibody immobilized on the insert is measured to determine the amount of antigen bound.

4. The process of claim 2 wherein step (d) comprises the steps of:

immersing the insert having antigen bound thereon in a second fluid containing a reagent capable of reacting specifically with the bound antigen, and

measuring the amount of reagent which has reacted with the bound antigen, in order to measure the amount of antigen bound, in order to measure the amount of antigen present in the fluid sample.

5. The process of claim 4 wherein the reagent comprises a radioactively tagged antibody.

6. The process of claim 1 wherein the fixed component is an enzyme and the mobile component is a substance capable of undergoing a chemical transformation catalyzed by the enzyme.

7. The process of claim 1 wherein the mobile component is an enzyme and the fixed component is a substance capable of undergoing a chemical transformation catalyzed by the enzyme.

8. The process of claim 1 wherein the solid phase insert matrix comprises a central rod and a plurality of fin-like projections extending outwardly from said rod along a portion of the length of said rod, said fins having outer edges conforming approximately to the shape of the fluid receptacle.

9. A process according to claim 1 wherein the solid phase insert matrix comprises a central rod and a plurality of fin-like projections extending outwardly from the rod along a portion of the length of the rod, said fins having a plurality of apertures therethrough providing increased total matrix surface area and having outer edges conforming approximately to the shape of the fluid receptacle.

10. A process according to claim 1 wherein the solid phase matrix comprises a central rod attached at one end to one face of a flat disc, at its center, said disc having attached on its opposite face a plurality of essentially equally spaced tines, oriented parallel to the axis of the central rod.

11. A process according to claim 1 wherein the solid phase matrix comprises a central rod at the axis and a plurality of concentric cylinders interconnected to each other and to the central rod by means of radial projections.

12. A process according to claim 1 wherein the solid phase matrix comprises a central rod at the axis and a continuous surface projecting therefrom in the form of a spiral ramp.

13. A process according to claim 1 wherein the solid phase matrix comprises a central rod at the axis and a plurality of uniformly spaced planar discs coaxial to the rod and to each other, the plane of said discs being approximately perpendicular to the central rod.

14. A method for producing a measurable reaction in a fluid sample in a reaction vessel comprising:

providing a fixed component immobilized on a solid phase matrix, said matrix comprising a handle member having attached at one end a plurality of elements having essentially smooth plane or curved surfaces so arranged with respect to one another and the handle member and of a size and shape such that when said matrix is inserted into said reaction vessel said matrix extends substantially throughout the depth of said fluid sample and the average diffusion distance of molecules in said fluid sample to the surfaces of said elements is greatly reduced relative to the average diffusion distance of said molecules in said fluid sample to the reaction vessel when no matrix is present therein, said fixed component being immobilized on the surfaces of said elements,

providing a mobile component capable of reacting measurably with the fixed component, and

placing the fixed component in reactive proximity with the mobile component, thereby producing a measurable reaction.

15. The method of claim 14 wherein the solid phase matrix comprises a central rod and a plurality of fin-like projections extending outwardly from said rod along a portion of the length of said rod.

16. The method of claim 14 wherein the solid phase matrix comprises a central rod attached at one end to one face of a flat disc, at its center, said disc having attached on its opposite face a plurality of essentially equally spaced times, oriented parallel to the axis of the central rod, said tines being sized to conform approximately to the shape of the fluid receptacle.

17. The matrix of claim 16 having means for individually attaching and detaching the tines.

18. The method of claim 14 wherein the solid phase matrix comprises a central rod at the axis and a plurality of concentric cylinders interconnected to each other and to the central rod by means of radial projections.

19. The method of claim 14 wherein the solid phase matrix comprises a central rod and a plurality of fin-like projections extending outwardly from the rod along a portion of the length of the rod, said fins having a plurality of apertures therethrough providing increased total matrix surface area and having outer edges conforming approximately to the shape of the fluid receptacle.

20. The method of claim 14 wherein the solid phase matrix comprises a central rod attached at one end to one face of a flat disc, at its center, said disc having attached on its opposite face a plurality of essentially equally spaced tines, oriented parallel to the axis of the central rod.

21. The method of claim 14 wherein the solid phase matrix comprises a central rod at the axis and a plurality of concentric cylinders interconnected to each other and to the central rod by means of radial projections.

22. The method of claim 14 wherein the solid phase matrix comprises a central rod at the axis and a continuous surface projecting therefrom in the form of a spiral ramp.

23. The method of claim 14 wherein the solid phase matrix comprises a central rod at the axis and a plurality of uniformly spaced planar discs coaxial to the rod and to each other, the plane of said discs being perpendicular to the central rod.

24. A solid phase matrix for insertion into a reaction vessel containing a fluid sample comprising molecules of a mobile component to be measured or detected, which matrix comprises, (1) a handle member having attached at one end a plurality of elements having essentially smooth plane or curved surfaces so arranged with respect to one another and the handle member and of a size and shape such that when said matrix is inserted into said reaction vessel said matrix extends substantially throughout the depth of said fluid sample and the average diffusion distance of said molecules of a mobile component to the surfaces of said elements is greatly reduced relative to the average diffusion distance of said molecules of a mobile component to the reaction vessel when no matrix is present therein, and (2) a fixed component immobilized on the surfaces of said elements, capable of reacting with said mobile component at a rate or to an extent measurable as a function of the concentration of said molecules of a mobile component.

25. The solid phase matrix of claim 24 comprising a central rod and a plurality of fin-like projections extending outwardly from said rod along a portion of the length of said rod.

26. The solid phase matrix of claim 24 comprising a central rod and a plurality of fin-like projections extending outwardly from the rod along a portion of the length of the rod, said fins having a plurality of apertures therethrough providing increased total matrix surface area relative to said projections lacking said apertures.

27. The solid phase matrix of claim 24 comprising a central rod attached at one end to one face of a flat disc, at its center, said disc having attached on its opposite face a plurality of essentially equally spaced tines, oriented parallel to the axis of the central rod.

28. The solid phase matrix of claim 24 comprising a central rod at the axis and a plurality of concentric cylinders interconnected to each other and to the central rod by means of radial projections.

29. The solid phase matrix of claim 24 comprising a central rod at the axis and a continuous surface projecting therefrom in the form of a spiral ramp.

30. The solid phase matrix of claim 24 comprising a central rod at the axis and a plurality of uniformly spaced planar discs coaxial to the rod and to each other, the plane of said discs being perpendicular to the central rod.

31. The solid phase matrix of claim 25 wherein the number of said fin-like projections ranges from 8 to 18.

32. The solid phase matrix of claim 25 wherein the number of said fin-like projections ranges from 16 to 18.

33. The solid phase matrix of claim 24 fabricated by a molding process using a mold with polished surfaces.

34. The solid phase matrix of claim 24 wherein the elements attached to the handle member are so dimensioned as to render the same size matrix suitable for use in reaction fluid volumes varying by at least 3-fold.

35. A solid phase matrix for insertion into a reaction vessel containing a fluid sample to be assayed for the presence of a given mobile component, which matrix comprises (1) a handle member having affixed thereto at one end a plurality of elements having essentially smooth plane or curved surfaces so arranged with respect to one another and the handle member, and of a size and shape such that the average diffusion distance between mobile component molecules and solid surface is greatly reduced relative to the average diffusion distance between mobile component molecules and the walls of the reaction vessel when no solid matrix is present;

(2) wherein each of said elements is substantially uniformly coated with a composition comprising a fixed component to which the mobile component being assayed for, if present, will adhere, the amount of said fixed component being so selected that adherence of the mobile component will occur at a rate measurable as a function of total concentration of mobile component in the fluid sample; and

(3) wherein the number, placement, size and shape of said elements is correlated with the amount of fixed component and the other physical and chemical characteristics of the coating composition to insure maximum availability of active fixed component at the surfaces of the elements.

36. The solid phase matrix of claim 35 comprising a central rod and a plurality of fin-like projections extending outwardly from said rod along a portion of the length of said rod.

37. The solid phase matrix of claim 35 wherein the number of said fin-like projections ranges from 8 to 18.

38. The solid phase matrix of claim 35 wherein the number of said fin-like projections ranges from 16 to 18.

39. The solid phase matrix of claim 35 fabricated by a molding process using a mold with polished surfaces.

40. The solid phase matrix of claim 35 wherein the elements attached to the handle member are so dimensioned as to render the same size matrix suitable for use in reaction fluid volumes varying by at least 3-fold.