Method and system for measuring blood coagulation time

• US 4,252,536 A
• Filed: 11/06/1978
• Issued: 02/24/1981
• Est. Priority Date: 11/12/1977
• Status: Expired due to Term

• Current Assignee: Kabushiki Kaisha Kyoto Daiichi Kagaku
• Original Assignee: Kabushiki Kaisha Kyoto Daiichi Kagaku
• Inventors: Yoshioka, Masahiro, Kishimoto, Shinichi
• Primary Examiner(s): Serwin, R. E.
• Application Number:
  US05/957,927
• Time in Patent Office: 841 Days
• Field of Search: 23/230 B, 23/918, 422/73, 356/39, 364/497, 364/498, 346/33 ME
• US Class Current: 356/36
• CPC Class Codes: G01N 21/272 for following a reaction, e.g. for determining photometrically a reaction rate (photometric cinetic analysis)
• CPC Class Codes: G01N 33/4905 Determining clotting time of blood by chemical methods G01N33/86, C12Q1/54

Abstract

A blood coagulation time measuring method comprising irradiating a mixture of blood plasma and reagent with a beam of specified amount of light, detecting changes in the amount of light scattered by the mixture, converting the resulting scattered light output signals to digital form at specified time interval, feeding the digital data to a computer, computing the difference between each input value and the input value immediately adjacent thereto with respect to time and storing the results, and determining the time, prior to the time at which a maximum difference value is obtained, at which a computed difference value corresponding to 1/n of the maximum difference value is obtained. The time thus determined is the end point of the coagulation time. A system for practicing this method is also disclosed.

Claims

1. A method for measuring the end point of blood coagulation time comprising the steps of

providing a mixture of a blood sample and a reagent,

irradiating the mixture with light.

detecting the amount of light scattered from the irradiated mixture and producing an electrical signal representative thereof, and

determining from said electrical signal a first time at which the most rapid change in the electrical signal is occurring and then determining as the end point at a time prior to said first time the time at which a change which is 1/n that of the most rapid change occurred, where n is greater than 1.

2. A method as in claim 1 wherein the step of detecting further comprises converting said electrical signal into digital signals at successive time intervals and storing the digital signals at succesive time intervals and storing the digital signals,

and the step of determining comprises comparing successively stored digital signals to determine the maximum difference between two successively stored digital signals as said first time at which the most rapid change is taking place.

3. A method as in claim 1 wherein n is an integer of 2 or more.

4. A system for measuring the end point of blood coagulation time comprising:

a cell for containing a mixture of blood plasma and a reagent,

means for irradiating the mixture with an amount of light,

means for detecting the amount of light scattered by the irradiated mixture and producing an electrical output signal representative thereof,

means for measuring at predetermined times the rate of change of said output signal corresponding to the rate of change of the scattered light,

means for determining a first time at which the maximum rate of change of the output signal takes place and for determining as the end point of the coagulation a time prior to said first time at which the rate of change of the output signal is 1/n that of the maximum rate of change, where n is greater than 1.

5. A system as in claim 4 where n is an integer in the range of 2 to 5.

6. A system as in claim 4 wherein said measuring means comprises means for converting said output signal at said predetermined times into digital signals, means for storing said digital signals, said determining means comparing successive stored digitial signals to determine said first time.

7. A system as in claim 6 wherein said determining means includes means for determining from the stored digital signals the time when the rate of change of the output signal is 1/n that of the maximum rate.

8. A system as in claim 4 further comprising switch means operatively related to said cell for starting the measuring upon placing the reagent into the cell.