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Miniature reaction container and a method and apparatus for introducing micro volumes of liquid to such a container

  • US 4,276,048 A
  • Filed: 06/14/1979
  • Issued: 06/30/1981
  • Est. Priority Date: 06/14/1979
  • Status: Expired due to Term
First Claim
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1. A method of conducting a chemical reaction with micro volumes of liquids, comprising the steps of:

  • (a) utilizing a reaction container including a tubular reaction chamber having an inlet tube at one end of said chamber substantially coaxial therewith, said inlet tube being of smaller diameter than said chamber, being in the nature of a capillary tube and being made from non-wettable material,(b) successively dipping said inlet tube of said container into a plurality of liquids,(c) at each dipping step, applying suction to the end of said reaction chamber opposite said inlet tube to suck a precise micro volume of each said liquid into said inlet tube and form successive coherent threads of said liquids therein,(d) removing said inlet tube from the last one of said liquids sucked into said inlet tube, and(e) applying further suction to said reaction chamber in at least one stage to control successive introduction of said threads of liquid into said reaction chamber and consequent mixing of said liquids within said reaction chamber.

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