Disposable sample card and method of making same

• US 4,301,414 A
• Filed: 10/29/1979
• Issued: 11/17/1981
• Est. Priority Date: 10/29/1979
• Status: Expired due to Term

• Current Assignee: United States Surgical Corporation (Medtronic Plc)
• Original Assignee: United States Surgical Corporation (Medtronic Plc)
• Inventors: Hill, Jeremy R., Meyer, Allen E.
• Primary Examiner(s): Krawczewicz, Stanley T.
• Application Number:
  US06/089,088
• Time in Patent Office: 750 Days
• Field of Search: 324/446, 324/441, 324/442, 324/447, 324/448, 324/450, 324/65 R, 324/64, 29/592 R, 29/876, 29/877, 128/637, 128/669, 73/344, 73/343.5
• US Class Current: 324/446
• CPC Class Codes: G01N 33/49 Blood chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80
• CPC Class Codes: G01R 27/22 Measuring resistance of fluids
• CPC Class Codes: Y10T 29/49002 Electrical device making
• CPC Class Codes: Y10T 29/49208 by assembling plural parts

Abstract

A disposable sample for use in measuring a parameter of a liquid sample such as the hematocrit and a method of making same. The disposable sample card is a micro-volume conductivity cell precision molded from plastic with built-in stainless steel electrodes. The sample card comprises a planar base portion on which is defined a capillary tube. First and second electrodes are disposed within the capillary tube in a spaced relationship and define a volume within the capillary tube. This volume constitutes a conductivity cell. The sample card may be used with an instrument which basically comprises an electronic portion for processing data obtained from the liquid sample on the sample card, a front-end mechanism for positioning the sample card within the instrument, and a digital display for displaying in eye-readable form the results of a parameter measurement made in the instrument.

Claims

1. A sample card for use in electrically operating on a liquid sample, the card comprising:

a body portion;

a capillary tube defined in said body portion for receiving a liquid sample;

first and second electrode means disposed within said capillary tube in a spaced relationship;

measurement cell means formed by said capillary tube and bounded by said first and second electrode means for housing a predetermined volume of said liquid sample; and

means adapted to operatively associate said first and second electrode means with an electrical signal.

2. The sample card of claim 1 further comprising entrance means for providing an entrance for said liquid sample into said capillary tube.

3. The sample card of claim 1, wherein said entrance means is a nozzle.

4. The saple card of claim 1, further comprising magnifying means for allowing a user to view an enlarged image of said measurement cell.

5. The sample card of claim 1, further comprising vent means for venting air in said capillary tube as said liquid sample is drawn into said tube by capillary action.

6. The sample card of claim 1, further comprising an evacuated portion in said body portion near said capillary tube.

7. The sample card of claim 1, further comprising guide means on said body portion, said guide means adapted to ensure proper orientation when said sample card is inserted in a device which electrically operates on said liquid sample.

8. The sample card of claim 1, further comprising registering means on said body portion, said registering means adapted to ensure proper registration when said sample card is positioned in a device which electrically operates on said liquid sample.

9. A blood sample carrier for use in measuring conductivity of a blood sample, said carrier comprising:

capillary tube means for receiving a blood sample;

first and second electrode means disposed within said capillary tube means in a spaced relationship;

measurement cell means, formed by said capillary tube means and bounded by said first and second electrode means, for segregating a predetermined volume of said liquid sample; and

means adapted to operatively associate said first and second electrode means with an electronic conductivity measuring circuit.

10. The blood sample carrier of claim 9, further comprising entrance means for providing an entrance for said liquid sample into said capillary tube means.

11. The blood sample carrier of claim 9, wherein said entrance means is a nozzle.

12. The sample card of claim 9, further comprising magnifying means for allowing a user to view an enlarged image of said measurement cell.

13. The sample card of claim 9, further comprising an evacuated portion in said body portion near said capillary tube means.

14. The sample card of claim 9, further comprising guide means on said body portion, said guide means adapted to ensure proper orientation when said sample card is inserted in a device which electrically operates on said liquid sample.

15. The sample card of claim 9, further comprising registering means on said body portion, said registering means adapted to ensure proper registration when said sample card is positioned in a device which electrically operates on said liquid sample.

16. A blood sample carrier for use in electrically measuring a parameter of a blood sample, said carrier comprising:

a planar base portion;

a hollow tube having an interior surface that defines a capillary tube on said base;

first and second electrodes disposed on a portion of said interior surface in a spaced relationship;

a measurement cell defined by said interior surface and said first and second electrodes, said measurement cell segregating a predetermined volume of said blood sample being measured; and

pad means, on said base, adpatd for electrically associating said first and second electrodes with a blood parameter measuring device.

17. The sample carrier of claim 16, wherein said interior surface of said capillary tube, when viewed in cross-section, is in the shape of a semicircle.

18. The sample carrier of claim 17, wherein said first electrode is disposed along the flat portion of a first cross-sectional semicircle and said second electrode is disposed along the flat portion of a second cross-sectional semicircle, said first and second semicircles being substantially parallel to and spaced from each other.

19. The sample carrier of claim 16, wherein said pad means comprises a first electrically conductive disc positioned on said base and connected to said first electrode, and a second electrically conductive disc positioned on said base and connected to said second electrode.

20. The sample card of claim 16, further comprising vent means for venting air in said capillary tube as said liquid sample is drawn into said tube by capillary action.

21. The sample card of claim 16, further comprising an evacuated portion in said body portion near said capillary tube.

22. The sample card of claim 16, further comprising guide means on said body portion, said guide means adapted to ensure proper orientation when said sample card is inserted in a device which electrically operates on said liquid sample.

23. The sample card of claim 16, further comprising registering means on said body portion, said registering means adapted to ensure proper registration when said sample card is positioned in a device which electrically operates on said liquid sample.

24. A method of making a disposable liquid sample carrier comprising the steps of:

forming a portion of a base member into a portion of the interior surface of a capillary tube;

providing an overlay having a surface;

positioning and securing two electrodes in a spaced relationship on said surface of said overlay; and

positioning and securing said overlay to said base member so that said surface of said overlay completes the interior surface of said capillary tube and so that said two electrodes along with the interior surface of said capillary tube segregates a volume constituting a measurement cell.

25. The method of claim 24, further comprising the step of configuring said interior surface of said capillary tube so that its transverse section resembles a semicircle.

26. The method of claim 25, wherein said providing step comprises providing an overlay having a substantially planar surface.

27. The method of claim 24, further comprising the step of forming a nozzle at an end of said capillary tube.

28. The method of claim 27, wherein said nozzle forming step includes forming a portion of the interior surface of said nozzle from said base member and the remainder of the interior surface of said nozzle from said overlay.

29. The method of claim 24, further comprising the step of making said base member from a translucent material.

30. The method of claim 29, wherein said translucent material is selected from the group consisting of polycarbonate, polymethacrylate, and styrene-butadiene resin.

31. The method of claim 24, further comprising the step of making said overlay from a translucent material.

32. The method of claim 24, further comprising the step of making said overlay from a Mylar.

33. The method of claim 24, further comprising the step of making said electrodes of stainless steel alloy.

34. The method of claim 24, wherein said electrode positioning and securing step includes securing a stainless steel alloy sheet to said overlay and etching away all of said stainless steel sheet except the portion defining said electrodes.

35. The method of claim 34, wherein said etching step is a photoetching step.

36. The method of claim 24, wherein said overlay is secured to said base member by employing an adhesive.

37. The method of claim 24, wherein said electrodes are secured to said overlay by employing an adhesive.

38. The method of claim 24, further comprising the step of forming a guide member on said base member.