Multi-analyte test device

• US 4,323,536 A
• Filed: 02/06/1980
• Issued: 04/06/1982
• Est. Priority Date: 02/06/1980
• Status: Expired due to Term

• Current Assignee: Clinical Diagnostic Systems Inc.
• Original Assignee: Eastman Kodak Company
• Inventors: Columbus, Richard L.
• Primary Examiner(s): Turk, Arnold
• Application Number:
  US06/118,840
• Time in Patent Office: 790 Days
• Field of Search: 356/244, 356/246, 23/230 B, 23/230 R, 422/55, 422/56, 422/57, 422/58, 422/100, 422/102, 261/112
• US Class Current: 422/412
• CPC Class Codes: B01L 3/502 with fluid transport, e.g. in multi-compartment structures centrifugal-type cuvettes G01N21/07; analysis by separation into components G01N30/00; automatic analysers G01N35/00
• CPC Class Codes: G01N 33/525 Multi-layer analytical elements

Abstract

A test device, and a method of testing, that use a plurality of test elements each for a different analyte, all elements being supplied analyte from a single quantity of liquid. The liquid is conveyed to the test elements by a capillary transport zone, and means for venting the zone are provided. To prevent contamination, liquid flow control means are included so that the liquid is confined to a plurality of flow paths each of which extends to only one test element.

Claims

1. A multi-analyte test device for the analysis of a plurality of analytes in a liquid, the device comprising,

a first member,

a second covering member, said members having opposing surfaces,

means for spacing said members apart a distance effective to induce capillary flow of liquid introduced between said surfaces and to create a liquid transport zone,

at a first location, access means permitting introduction of a quantity of liquid into said zone, said access means being in either said first or second members or said spacing means, said first or second member opposite to said access means being configured to create a temporary energy barrier to liquid flow beyond the vicinity of said access means;

a plurality of individual test elements disposed on one of said members at locations other than said first location and spaced away from the other of said members, said other member in the areas directly opposite to said test elements being recessed, at least two of said elements each respectively comprising a test composition for the generation of a detectable response indicative of a different one of the analytes, and

control means for confining liquid flow from said access means to a plurality of predetermined flow paths each of which extends to only one of said test elements,

whereby all of said analytes from a single quantity of the liquid are each detectable by the respective test element for that analyte without contamination from others of said test elements.

4. A device as defined in claim 1 or 2, wherein said test elements each comprise an absorbent layer containing at least one reagent, and said test compositions react with the analytes of the liquid to generate a radiometrically detectable response.

5. A device as defined in claim 1 or 2, wherein said each test element includes all the reagents necessary for a radiometric assay of a particular analyte.

6. A device as defined in claim 1 or 2, wherein said two elements include, respectively, the reagents necessary for the detection in said elements of total protein and blood urea nitrogen.

2. A multi-analyte test device for the analysis of a plurality of analytes in a liquid, the device comprising,

a first member,

a second covering member, said members having opposing surfaces,

means for spacing said members apart a distance effective to induce capillary flow of liquid introduced between said surfaces and to create a liquid transport zone,

at a first location, access means permitting introduction of a quantity of liquid into said zone, said access means being in either said first or said second member, the portion of said second or first of said members generally opposite to said access means being a non-test area;

a plurality of individual test elements disposed on said opposite member around said non-test area and spaced away from the other of said members, at least two of said elements each respectively comprising a test composition for the generation of a detectable response indicative of a different one of the analytes, and

control means for confining liquid flow from said access means to a plurality of predetermined flow paths each of which extends to only one of said test elements, said control means including means for preventing introduced liquid from flowing onto said test elements until after said non-test area is completely wetted.

3. A device as defined in claim 2, wherein said control means includes a plurality of vent means for venting air from said zone ahead of advancing liquid, a vent means being associated with each of said test elements.