Gonadostatin
First Claim
1. A method of deriving substantially pure gonadostatin, a proteinaceous mammalian gonadal substance characterized by its selective inhibition of LRF-stimulated pituitary secretions of LH and FSH in dosages below a threshold level and general stimulation of pituitary hormone secretion in dosages above a threshold level, the method comprising:
- obtaining a mammalian gonadal secretion;
initially separating the components of said secretion according to molecular weight by gel filtration and obtaining a partially purified material containing eluate fractions which deomonstrate gonadostatin activity;
further separating components of said partially purified material by partition chromatography with a two-phase 1-butanol, pyridine, acetic acid, water eluent system to obtain further purified material; and
additionally purifying said further purified material by reverse phase high performance liquid chromatography with a formic acid, pyridine, 1-propanol, water eluent system of increasing 1-propanol concentration to obtain a substantially purified substance which demonstrates activity in inhibiting the LRF-stimulated secretion of LH and FSH by the pituitary.
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Accused Products
Abstract
A substantially purified substance has been isolated from bovine gonadal secretions which mediates pituitary hormone secretion, using an extraction and purification sequence that includes gel filtration, partition chromatography and HPLC in that order. The substance, designated gonadostatin, is a moderate-size peptide which below a threshold level selectively inhibits LRF-stimulated pituitary secretion of LH and FSH. Above a threshold level, gonadostatin generally stimulates pituitary hormone secretion.
10 Citations
5 Claims
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1. A method of deriving substantially pure gonadostatin, a proteinaceous mammalian gonadal substance characterized by its selective inhibition of LRF-stimulated pituitary secretions of LH and FSH in dosages below a threshold level and general stimulation of pituitary hormone secretion in dosages above a threshold level, the method comprising:
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obtaining a mammalian gonadal secretion; initially separating the components of said secretion according to molecular weight by gel filtration and obtaining a partially purified material containing eluate fractions which deomonstrate gonadostatin activity; further separating components of said partially purified material by partition chromatography with a two-phase 1-butanol, pyridine, acetic acid, water eluent system to obtain further purified material; and additionally purifying said further purified material by reverse phase high performance liquid chromatography with a formic acid, pyridine, 1-propanol, water eluent system of increasing 1-propanol concentration to obtain a substantially purified substance which demonstrates activity in inhibiting the LRF-stimulated secretion of LH and FSH by the pituitary. - View Dependent Claims (2, 3, 4, 5)
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Specification