Tagged immunoassay
First Claim
1. An immunoassay method for measurement of the content of a target antigen or antibody in a fluid or tissue specimen, which comprises reacting the target with reagent antibody or antigen which forms a complex with the target and is tagged with tagging elements or compounds which are unassociated chemically with said reagent and are protected against reaction with the target and the biological and chemical environment of the assay, and detecting the formed, tagged complexes.
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Accused Products
Abstract
An immunoassay method for measurement of the content of a target antigen or antibody in a fluid or tissue specimen comprises reacting the target with reagent antibody or antigen which forms a complex with the target and is tagged with tagging elements which are unassociated chemically with said reagent and are protected against reaction with the target and the biological and chemical environment of the assay. Preferably the reagent antibody or antigen is carried by small, tagged mobile units, such as latex particles of a size smaller than 0.8 μm, having tagging elements embedded therein. The tagged complexes which are formed may be measured by X-ray fluorescence or by detection of radioactive decay. Different target antigens or antibodies can be assayed simultaneously by employing different tagged mobile units, and the mobile units with the tagging elements can be recovered for disposal or for reuse.
54 Citations
46 Claims
- 1. An immunoassay method for measurement of the content of a target antigen or antibody in a fluid or tissue specimen, which comprises reacting the target with reagent antibody or antigen which forms a complex with the target and is tagged with tagging elements or compounds which are unassociated chemically with said reagent and are protected against reaction with the target and the biological and chemical environment of the assay, and detecting the formed, tagged complexes.
- 10. An immunoassay method for measurement of the content of a target antigen or antibody in a fluid or tissue specimen, which comprises reacting the target with reagent antibody or antigen which forms a complex with the target and is carried by small tagged mobile units having tagging elements or compounds which are unassociated chemically with said reagent and are protected against reaction with the target and the biological and chemical environment of the assay, and detecting the tagged mobile units bearing formed complexes.
- 24. An immunoassay method for measurement of the content of a target antigen or antibody in a fluid or tissue specimen, which comprises coupling reagent antibody or antigen, which forms a complex with the target, with small tagged mobile units having tagging elements or compounds which are unassociated chemically with said reagent and are protected against reaction with the target and the biological and chemical environment of the assay, reacting the tagged mobile units coupled with antibody or antigen with the target antigen or antibody, separating the unreacted mobile units and measuring the number of reacted mobile units by detection of the tagging elements or compounds.
Specification