Analytical device having semiconductive organic polymeric element associated with analyte-binding substance

• US 4,444,892 A
• Filed: 05/17/1982
• Issued: 04/24/1984
• Est. Priority Date: 10/20/1980
• Status: Expired due to Term

• Current Assignee: Omicron Technologies, Inc. (Biomedical Research Foundation of Northwest Louisiana)
• Original Assignee: Mark K. Malmros
• Inventors: Malmros, Mark K.
• Primary Examiner(s): Marantz, Sidney
• Application Number:
  US06/378,399
• Time in Patent Office: 708 Days
• Field of Search: 436/518, 436/531, 436/528, 436/531, 436/806, 436/827, 324/71 SN
• US Class Current: 436/528
• CPC Class Codes: C12Q 1/00 Measuring or testing processes involving enzymes, nucleic acids or microorganisms measuring or testing apparatus with condition measuring or sensing means, e.g. colony counters, C12M1/34; Compositions therefor; Processes of preparing such compositions
• CPC Class Codes: G01N 33/542 with steric inhibition or signal modification, e.g. fluorescent quenching
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: Y10S 436/806 Electrical property or magnetic property
• CPC Class Codes: Y10S 436/827 Lectins

Abstract

A method, sensor and semiconductor device for determining the concentration of an analyte in a medium. The device features an element constructed of semiconductive organic polymer associated with a binding substance having specific affinity for the analyte.

Claims

1. A variable resistance sensor for the determination of an analyte in a medium comprising:

(a) an insulating structure,

(b) element means having an active part comprised of semiconductive organic polymer of polyacetylene including a specific binding substance for the analyte, mounted on said structure for exposure to the medium and

(c) electrodes positioned to contact the element.

2. The sensor as in claim 1 wherein there are four electrodes.

3. The sensor as in claim 1 where there are two electrodes.

4. The sensor as in claim 1 where there are three electrodes.

5. The sensor as in claims 1 or 4 wherein the specific binding substance is selected from the group consisting of antibodies, antigens, enzymes, enzyme substrates, analogs of enzyme substrates, agglutinins, lectins, enzyme cofactors, enzyme inhibitors and hormones.

6. A method of identifying the type and quantity of an analyte in a medium comprising the steps of:

(a) preparing an element of semiconductive organic polymer of polyacetylene

(b) treating the element with a specific binding substance,

(c) exposing the element to the medium,

(d) measuring the change in an electrical characteristic of the treated element.

7. The method of claim 6 including the steps of applying at least two electrodes to contact the element and measuring the electrical changes between the electrodes.

8. The method of claim 7 including the step of applying three electrodes to contact the element.

9. The method of claims 6, 7 or 8 where the specific binding substance is selected from the group consisting of an antibody, an antigen, an enzyme, an enzyme substrate, an enzyme substrate analog, an agglutinin or lectin, an enzyme cofactor, an enzyme inhibitor and a hormone.