Water-insoluble protein material, its preparation and its use

• US 4,478,976 A
• Filed: 09/20/1982
• Issued: 10/23/1984
• Est. Priority Date: 09/25/1981
• Status: Expired due to Fees

• Current Assignee: BASF AG (BASF SE)
• Original Assignee: BASF AG (BASF SE)
• Inventors: Sanner, Axel, Goertz, Hans-Helmut, Marcinowski, Stefan
• Primary Examiner(s): Kight, John
• Assistant Examiner(s): Nutter, Nathan M.
• Application Number:
  US06/420,317
• Time in Patent Office: 764 Days
• Field of Search: 525/54.1, 527/200, 527/201, 527/207, 435/174, 435/177, 435/180, 427/2, 427/414
• US Class Current: 525/54.1
• CPC Class Codes: C12N 11/082 obtained by reactions only involving carbon-to-carbon unsaturated bonds
• CPC Class Codes: C12N 11/087 Acrylic polymers

Abstract

A biologically active protein which is bonded to a water-insoluble porous copolymer based on N-vinylimidazole and/or substituted N-vinylimidazoles and monomers which can be copolymerized therewith, its preparation and its use for carrying out enzymatic reactions.

Claims

1. A water-insoluble protein material consisting essentially of a biologically active protein on a water-insoluble carrier, wherein the protein is adsorbed onto a water-insoluble copolymer based on 30 to 99 percent, by weight of the copolymer, of N-vinylimidazole or substituted N-vinylimidazoles, 1 to 70 percent by weight of a copolymerizable, polyolefinically unsaturated monomer and 0 to 69 percent by weight of monomers which are copolymerizable therewith.

2. The protein material of claim 1, wherein the copolymer is a bead polymer.

3. The protein material of claim 1 or 2, wherein the carrier contains, as the copolymerizable monomer, a polyolefinically unsaturated component.

4. The protein material of claim 3, wherein the polyunsaturated component of the carrier is divinylstyrene, a polyfunctional ester of acrylic acid or methacrylic acid, a polyolefinically unsaturated urea derivative or a polyolefinically unsaturated acid amide.

5. The protein material of claim 4, wherein the carrier contains, as a further comonomer, styrene, a (C_1-4 -alkyl)-styrene, acrylic acid or methacrylic acid or an alkyl or aminoalkyl ester thereof or a vinylheterocyclic compound.