Method of making an electrochemical sensing cell

• US 4,534,825 A
• Filed: 03/09/1984
• Issued: 08/13/1985
• Est. Priority Date: 03/10/1980
• Status: Expired due to Term

• Current Assignee: Cordis Europa NV (Johnson & Johnson)
• Original Assignee: Cordis Europa NV (Johnson & Johnson)
• Inventors: Bergveld, Piet, Koning, Gerrit
• Primary Examiner(s): Kimlin, Edward
• Assistant Examiner(s): Hoch, Ramon R.
• Application Number:
  US06/587,819
• Time in Patent Office: 522 Days
• Field of Search: 156/644, 156/652, 156/650, 156/625, 156/659.1, 156/654, 156/656, 156/657, 29/570, 29/580, 29/590, 29/591, 29/578, 204/400, 204/403, 204/1 T, 204/418, 204/429, 204/416, 357/25, 128/635
• US Class Current: 438/49
• CPC Class Codes: A61B 5/14542 for measuring blood gases A61B5/14551 takes precedence
• CPC Class Codes: A61B 5/1473 invasive, e.g. introduced into the body by a catheter A61B5/1482 takes precedence
• CPC Class Codes: A61B 5/1495 Calibrating or testing of in-vivo probes
• CPC Class Codes: G01N 27/28 Electrolytic cell components
• CPC Class Codes: G01N 27/4163 checking the operation of, or calibrating, the measuring apparatus G01N27/3274, G01N27/4175 and G01N33/0006 take precedence
• CPC Class Codes: G01N 27/4165 for pH meters

Abstract

An in vivo electrochemical monitoring device is formed by a catheter-like member which terminates in a closed end having a wall with a fixed opening to admit fluid to be tested, such as blood in an artery. An electrochemical sensor, such as an ISFET device for monitoring the concentration of a particular ion in blood, is mounted inside the tube at a fixed location below the opening preferably a larger sensing chamber. An infusion channel in the tube is arranged to flood the sensor with a fluid of known chemical properties so that the sensor output can be calibrated. Under pressure the calibration fluid expels the test fluid out of the tube or chamber via the fixed opening. A method of constructing a suitable chamber on an ISFET wafer is also disclosed.

Claims

1. A method of making a sensing cell for a chemically sensitive probe for measuring the properties of biological fluids in vivo, comprising the steps of

producing a wafer comprising an ISFET with spaced source and drain regions spanned by an ion-sensitive gate portion,

covering said source and drain regions and gate portion of said ISFET with a temporary first layer of soluble material,

covering said temporary first layer with a second layer of relatively insoluble material,

defining an opening in said second layer directly above said gate region, and

removing said temporary first layer which covers the source and drain regions and the gate portion by introducing a solvent through said opening to dissolve said first layer, thus forming a sensing chamber encompassing the source and drain regions as well as the gate portion of said ISFET.

2. The method of claim 1, wherein said second layer is electrically conductive.

3. The method of claim 1, further comprising the step of forming an infusion through-hole through the wafer leading into said sensing chamber,

whereby a known fluid can be introduced through said through-hole into said chamber for calibration in vivo.

4. The method of claim 1, wherein said opening formed in said second layer is narrower than said chamber formed by removing said first layer.

5. The method of claim 1, further comprising the step of covering said cell with a layer of hydrogel to promote biological compatability.