Process for measuring fluorescence

• US 4,549,807 A
• Filed: 10/07/1983
• Issued: 10/29/1985
• Est. Priority Date: 10/07/1983
• Status: Expired due to Term

• Current Assignee: Timothy Dean Harris
• Original Assignee: AT&T, Inc.
• Inventors: Hoffmaster, Timothy D.
• Primary Examiner(s): EVANS, FANNIE L
• Application Number:
  US06/539,793
• Time in Patent Office: 753 Days
• Field of Search: 356/301, 356/317, 356/318, 356/417, 250/365, 250/372, 250/373, 250/458.1, 250/459.1, 250/461.1, 250/461.2, 436/172
• US Class Current: 356/318
• CPC Class Codes: G01N 2021/6432 Quenching
• CPC Class Codes: G01N 2021/6439 with indicators, stains, dyes, tags, labels, marks
• CPC Class Codes: G01N 21/648 using evanescent coupling or surface plasmon coupling for the excitation of fluorescence

Abstract

Fluorescent measurements are taken utilizing a Raman enhancing surface by a method which allows reduction or essentially complete elimination of interference by fluorescence quenchers. In particular, the fluorescent medium to be measured is interacted with a Raman enhancing surface. Through this interaction between the Raman enhancing surface and the fluorescing species, fluorescence quenching is eliminated. The quenching is particularly significant in analytical procedures such as those involved in chromatography and medical testing.

Claims

1. A process for determining the presence of a fluorescing species in a condensed phase sample that interacts with an entity that quenches said fluorescence, said process comprising the steps of exciting said fluorescing species with electromagnetic radiation and monitoring the electromagnetic radiation emanating from said sample wherein said species in said sample is allowed to interact with a Raman enhancing surface during said determination.

2. The process of claim 1 wherein said monitoring is accomplished with a photomultiplier.

3. The process of claim 1 wherein said sample is a liquid.

4. The process of claim 1 wherein said sample is a solid.

5. The process of claim 1 wherein said exciting electromagnetic radiation is coherent.

6. The process of claim 1 wherein said exciting electromagnetic radiation is incoherent.

7. The process of claim 1 wherein said Raman enhancing surface comprises a metal chosen from the group consisting of silver, copper, and gold.