Enzyme electrode and method for dextran analysis
First Claim
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1. An electrode for the potentiometric determination of dextran in a solution comprising a platinum redox electrode, a first cellulose absorbent sheath surrounding the electrode with immobilized glucose oxidase, a second absorbent sheath thereover with immobilized enzymes selected from the group consistinng of α
- -glucosidase and isomaltase, with the provision that a means for excluding compounds having a molecular weight of 14,000 or greater surrounding said second sheath is included when α
-glucosidase is selected and when the solution contains starch and an outer absorbent sheath with immobilized dextranase surrounding said means wherein the amounts of glucose oxidase, α
-glucosidase or isomaltase and dextranase are sufficient to determine the amount of dextran.
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Abstract
A method for the potentiometric determination of a dextran solution is provided wherein the dextran is enzymatically hydrolyzed to glucose, the glucose is oxidized to form hydrogen peroxide and the hydrogen peroxide is measured utilizing a redox electrode. A novel electrode having a plurality of enzyme impregnated layers is provided for converting the dextran to glucose.
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12 Claims
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1. An electrode for the potentiometric determination of dextran in a solution comprising a platinum redox electrode, a first cellulose absorbent sheath surrounding the electrode with immobilized glucose oxidase, a second absorbent sheath thereover with immobilized enzymes selected from the group consistinng of α
- -glucosidase and isomaltase, with the provision that a means for excluding compounds having a molecular weight of 14,000 or greater surrounding said second sheath is included when α
-glucosidase is selected and when the solution contains starch and an outer absorbent sheath with immobilized dextranase surrounding said means wherein the amounts of glucose oxidase, α
-glucosidase or isomaltase and dextranase are sufficient to determine the amount of dextran. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
- -glucosidase and isomaltase, with the provision that a means for excluding compounds having a molecular weight of 14,000 or greater surrounding said second sheath is included when α
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9. A method for the potentiometric determination of a dextran in a solution comprising immersing into a sample, an electrode containing a platinum redox electrode, a first cellulose absorbent sheath surrounding the electrode with immobilized glucose oxidase, a second absorbent sheath thereover with immobilized enzymes selected from the group consisting of α
- -glucosidase and isomaltase, with the provision that a means for excluding compounds having a molecular weight of 14,000 or greater surrounding said second sheath is included when α
-glucosidase is selected and when the solution contains starch and an outer absorbent sheath with immobilized dextranase surrounding said means wherein the amounts of glucose oxidase, α
-glucosidase or isomaltase and dextranase are sufficient to determine the amount of dextran, incubating and measuring the voltage which correlates with the amount of dextran present. - View Dependent Claims (10, 11, 12)
- -glucosidase and isomaltase, with the provision that a means for excluding compounds having a molecular weight of 14,000 or greater surrounding said second sheath is included when α
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