Sensor element for fluorescence-optical measurement

• US 4,568,518 A
• Filed: 12/02/1983
• Issued: 02/04/1986
• Est. Priority Date: 12/06/1982
• Status: Expired due to Fees

• Current Assignee: AVL AG Grabenstrasse 11 Schaffhausen
• Original Assignee: AVL AG
• Inventors: Offenbacher, Helmut, Wolfbeis, Otto, Kroneis, Herbert
• Primary Examiner(s): NOT, DEFINED
• Application Number:
  US06/557,209
• Time in Patent Office: 795 Days
• Field of Search: 422/56, 422/57, 436/172, 436/163, 436/169, 428/290, 427/2, 427/157, 427/162
• US Class Current: 422/420
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/80 Indicating pH value

Abstract

Fluorescence-optical indicator material is chemically bonded in a network structure permeating a carrier membrane of the sensor element. As this network structure is independent of the membrane and not chemically bonded thereto, the properties of the membrane and the indicator are not affected by the immobilization.

Claims

1. A flexible sensor element useful in making fluorescence-optical measurements, said sensor element comprising a carrier membrane and an immobilized network structure integrated within said carrier membrane, said immobilized network structure including a fluorescent indicator material, said fluorescent indicator material being non-chemically bonded to said carrier membrane.

2. A flexible sensor element as defined in claim 1, wherein said carrier membrane consists of cellulose.

3. A flexible sensor element as defined in claim 1, wherein said flexible sensor element consists of said carrier membrane and said immobilized network structure integrated within said carrier membrane.

4. A flexible sensor element useful in making fluorescence-optical measurements, said sensor element comprising a carrier membrane and an immobilized network structure extending within said carrier membrane, said immobilized network structure including a fluorescent indicator material, said fluorescent indicator material being non-chemically bonded to said carrier membrane, said flexible sensor element being made by the steps of

(a) providing a carrier membrane,

(b) soaking said carrier membrane in a solution of cross-linking material until said carrier membrane is permeated with said cross-linking material,

(c) adding a fluorescent indicator material to said carrier membrane in step (b), and

(d) allowing said network structure containing said fluorescent indicator material to form within said carrier membrane.