Method for detecting biological activity in a body fluid

• US 4,632,902 A
• Filed: 01/03/1985
• Issued: 12/30/1986
• Est. Priority Date: 08/20/1981
• Status: Expired due to Term

• Current Assignee: Becton, Dickinson & Co
• Original Assignee: Becton, Dickinson & Co
• Inventors: Broman, Rodney, Waters, John R.
• Primary Examiner(s): Rosen, Sam
• Assistant Examiner(s): Herald, William J.
• Application Number:
  US06/688,276
• Time in Patent Office: 726 Days
• Field of Search: 210/679, 435/29, 435/31, 435/32, 435/33, 435/34, 435/36, 435/37, 435/38, 435/253, 435/254, 435/255, 435/803, 435/261, 435/245, 435/244
• US Class Current: 435/29
• CPC Class Codes: C12Q 1/16 using radioactive material
• CPC Class Codes: Y10S 435/803 Physical recovery methods, e.g. chromatography, grinding

Abstract

A method of detections biological activity is disclosed. A nutrient growth medium which isolates antibiotics and other microbial growth inhibitors during culturing of a microorganism. The growth medium includes an aqueous dispersion of nutrient materials and an effective amount of an isolating substance or substances capable of isolating antimicrobial materials during culturing of a microorganism. The isolating substances are selected from ion exchange resins and non-functional adsorbent resins.

Claims

1. A method for detecting biological activity in a body fluid sample which may contain antimicrobial material wherein:

(a) a growth medium is inoculated with a body fluid sample

(b) the inoculated growth is cultured under conditions conducive to the occurrence of normal metabolic processes for a period of time sufficient to cause production of metabolic byproducts, and

(c) the metabolic byproducts are monitored to detect biological activity,

(d) the improvement comprising incorporating into said growth medium an effective amount of a resin capable of isolating any antimicrobial material present in said body fluid sample without hindering biological activity during said culturing period.

2. The method of claim 1 wherein said resin is an ion exchange resin.

3. The method of claim 1 wherein said resin is a non-functional resin adsorbent.

4. The method of claim 1 wherein said resin is a non-functional resin adsorbent in combination with an ion exchange resin.

5. The method of claim 2 or 4 wherein said ion exchange resin is a cationic exchange resin.

6. The method of claim 5 wherein the cationic exchange resin is in the sodium form.

7. The method of claim 1 wherein the resin is selected from the group consisting of a non-functional copolymer of divinyl benzene and styrene, an ion exchange resin which is a copolymer of divinyl benzene and styrene and combinations thereof.

8. The method of claim 1 wherein the body fluid sample is blood.

9. The method of claim 1 wherein the body fluid sample is urine.

10. The method of claim 1 wherein the body fluid sample is spinal fluid.

11. The method of claim 1 wherein the body fluid sample is peritoneal fluid.

12. The method of claim 1 wherein said resin is detergent coated.