Genes encoding bovine prolactin

• US 4,675,297 A
• Filed: 09/14/1983
• Issued: 06/23/1987
• Est. Priority Date: 02/23/1981
• Status: Expired due to Term

• Current Assignee: Regents of the University of California (University of California)
• Original Assignee: Regents of the University of California (University of California)
• Inventors: Martial, Joseph A., Miller, Walter L., Baxter, John D.
• Primary Examiner(s): Martinell, James
• Application Number:
  US06/532,107
• Time in Patent Office: 1,378 Days
• Field of Search: 435/68, 435/70, 435/91, 435/172.3, 435/253, 435/317, 435/243, 536/27, 935/19, 935/21, 935/13, 935/29, 935/73
• US Class Current: 435/252.33
• CPC Class Codes: C07K 14/57554 Prolactin
• CPC Class Codes: C07K 2319/00 Fusion polypeptide
• CPC Class Codes: C07K 2319/02 containing a signal sequence
• CPC Class Codes: C07K 2319/75 containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Abstract

A DNA sequence encoding bovine prolactin and optionally including condons for the preceding 10 amino acids is used to construct expression systems to obtain recombinant production of these proteins.

Claims

1. A DNA which comprises a recombinant DNA sequence encoding a bovine prolactin of the amino acid sequence ##STR15##

2. The DNA of claim 1 which further comprises a deoxyribonucleotide sequence encoding the amino acid sequence ##STR16## so that said DNA encodes the amino acid sequence ##STR17##

3. A DNA transfer vector which comprises the DNA of claim 1.

4. A DNA transfer vector which comprises the DNA of claim 2.

5. A microorganism transformed by the transfer vector of claim 3.

6. A microorganism transformed by the transfer vector of claim 4.

7. A DNA expression vector which comprises the DNA of claim 1.

8. A DNA expression vector which comprises the DNA of claim 2.

9. A microorganism transformed by the expression vector of claim 7.

10. A microorganism transformed by the expression vector of claim 8.

11. The microorganism of claim 5 or 6 comprising Escherichia coli χ

1776.

12. The microorganism of claim 9 or 10 comprising Escherichia coli χ

1776 or Escherichia coli HB101.