Solid phase assay with visual readout

• US 4,703,017 A
• Filed: 02/14/1984
• Issued: 10/27/1987
• Est. Priority Date: 02/14/1984
• Status: Expired due to Term

• Current Assignee: Becton, Dickinson & Co
• Original Assignee: Becton, Dickinson & Co
• Inventors: Campbell, Robert L., O'Connell, James P., Wagner, Daniel B.
• Primary Examiner(s): Marantz, Sidney
• Assistant Examiner(s): Saunders, David A.
• Application Number:
  US06/579,667
• Time in Patent Office: 1,351 Days
• Field of Search: 422/55, 422/56, 422/57, 422/61, 424/7.1, 435/7, 435/805, 435/810, 436/501, 436/823, 436/827, 436/828, 436/501, 436/520, 436/525, 436/529, 436/530, 436/533, 436/534, 436/800, 436/801, 436/808, 436/829
• US Class Current: 436/501
• CPC Class Codes: B82Y 5/00 Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• CPC Class Codes: G01N 33/532 Production of labelled immunochemicals
• CPC Class Codes: G01N 33/543 with an insoluble carrier for immobilising immunochemicals
• CPC Class Codes: Y10S 436/80 Fluorescent dyes, e.g. rhodamine
• CPC Class Codes: Y10S 436/808 Automated or kit
• CPC Class Codes: Y10S 436/809 Multifield plates or multicontainer arrays
• CPC Class Codes: Y10S 436/815 Test for named compound or class of compounds
• CPC Class Codes: Y10S 436/818 Human chorionic gonadotropin
• CPC Class Codes: Y10S 436/829 Liposomes, e.g. encapsulation

Abstract

Solid phase assay for an analyte wherein binder is supported on a solid support, such as nitrocellulose, and the tracer is comprised of ligand labeled with a colored particulate label, such as a liposome including a dye. The assay has a high sensitivity, and the tracer is visible on the support under assay conditions, whereby tracer can be determined, without instrumentation, and without further treatment thereof.

Claims

1. A process for assaying for an analyte, comprising:

contacting a binder supported on a test area of a solid with a solution of analyte and a tracer, said binder being a binder for at least the analyte, said tracer being comprised of a ligand labeled with a visible particulate label wherein when said particulate label is a sac including a visable dye, said sac is selected from the group consisting of liposomes and microcapsules, said ligand being bound to one of the binder and analyte whereby the tracer is bound to one of the binder on the support and the analyte bound to the binder on the support, said test area being formed of a material having a surface area for supporting the binder and the binder being supported in a concentration whereby bound tracer is visible on the support under assay conditions, said binder being supported in a concentration of at least 1 μ

g/cm² ; and

determining the visibility of tracer bound in said test area as a measure of analyte in a sample.

2. The process of claim 1 wherein analyte and tracer are sequentially contacted with the binder on the solid support.

3. The process of claim 1 wherein the analyte and tracer are simultaneously contacted with binder on the solid support.

4. The process of claim 1 wherein the label of the tracer is a sac including a visible dye.

5. The process of claim 4 wherein the sac is a liposome.

6. The process of claim 5 wherein the visibility of the bound tracer is determined without lysis of the liposome.

7. The process of claim 1 wherein the solid support is in sheet form.

8. The process of claim 1 wherein the binder is an antibody.

9. The process of claim 8 wherein the tracer is a labeled form of the analyte.

10. The process of claim 8 wherein the tracer is a labeled form of an antibody for the analyte.

11. The process of claim 1 wherein the analyte is digoxin.

12. The process of claim 1 wherein the analyte is hCG.

13. The process of claim 1 wherein the particulate label is a colored particle.

28. The process of claim 1 wherein the assay is for hCG, the binder is antibody to hCG and said tracer is comprised of antibody to hCG labeled with a colored particulate label.

29. The process of claim 28 wherein the test area is formed of nitrocellulose.

30. The process of claim 29 wherein the particulate label is a sac.

31. The process of claim 30 wherein the particulate label is a sac containing a visible dye and visibility is determined without lysis of the sac.

32. The process of claim 31 wherein the particulate label is a liposome containing rhodamine.

33. The process of claim 1 wherein the assay is for digoxin said binder is antibody to digoxin and the tracer is comprised of an analogue of digoxin bound by said antibody labeled with a colored particulate label.

34. The process of claim 33 wherein the test area is formed of nitrocellulose.

35. The process of claim 34 wherein the particulate label is a sac.

36. The process of claim 35 wherein the particulate label is a sac containing a visible dye and visibility is determined without lysis of the sac.

37. The process of claim 36 wherein the particulate label is a liposome containing rhodamine.

46. The process of claim 1 wherein the analyte is present in a concentration of no greater than 10^-9 grams per milliliter.

47. The process of claim 1 wherein said test area is capable of supporting binder in a concentration of at least ten micrograms per centimeter.

48. The process of claim 46 or 47 wherein the binder is in the test area as a spot.

49. The process of any one of claims 1, 46 or 48 wherein the particulate label is a precipitated or insoluble metal or metal alloy.

50. The process of claim 46 wherein the label of the tracer is a liposome including a visible dye.

56. The process of claim 1 wherein the ligand is bound to the analyte whereby the tracer is bound to the analyte.

57. The process of claim 56 wherein ligand is directly bound to the analyte whereby the tracer is directly bound to the analyte.

14. A process for assaying for an analyte, comprising:

contacting a binder supported on a test area of a solid support with a solution analyte and a tracer, said binder being a binder for at least the analyte, said tracer being comprised of a ligand labeled with a visible particulate label wherein when said particulate label is a sac including a visible dye, said sac is selected from the group consisting of liposomes and microcapsules, said ligand being bound by one of the binder on the support and the analyte bound to the binder on the support, said test area being formed of nitrocellulose, said binder being supported in said test area in a concentration whereby bound tracer is visible on the support under assay conditions, said binder being supported in a concentration of at least 1 μ

g/cm² ; and

determining the visibility of tracer bound in said test area as a measure of analyte in a sample.

15. The process of claim 14 wherein the visible particulate label is a sac including a visible dye.

16. The process of claim 15 wherein the sac is a liposome.

17. The process of claim 16 wherein the visibility of tracer in said test area is determined without lysis of the liposome.

18. The process of claim 17 wherein the analyte is hCG.

19. The process of claim 17 wherein the analyte is digoxin.

20. The process of claim 17 wherein the analyte is in a sample in a concentration of no greater than 10^-9 gm/ml.

21. The process of claim 14 wherein the analyte is in a sample in a concentration of no greater than 10^-9 gm/ml.

22. The process of claim 14 wherein the solid support is in sheet form.

23. The process of claim 14 wherein the support contains a plurality of said test areas.

24. The process of claim 14 wherein the particulate label is a colored particle.

25. The process of claim 14 wherein the particulate label is a hydrophobic dye.

26. The process of claim 14 wherein the binder is supported in a concentration of at least 40 μ

g/cm².

27. The process of claim 26 wherein the binder is in the test area as a spot having a diameter of from 3 to 5 mm.

51. The process of claim 14 or 21 wherein the particulate label is a precipitated or insoluble metal or metal alloy.

54. The process of claim 14 wherein the ligand is bound to the analyte whereby the tracer is bound to the analyte.

55. The process of claim 54 wherein ligand is directly bound to the analyte whereby the tracer is directly bound to the analyte.

58. The process of claim 5 or 16 wherein the visible dye is sulforhodamine B.

38. A reagent kit for determining an analyte in solution comprising:

a solid support including a binder on a test area of the solid support in a concentration of at least 1 μ

g/cm² ; and

a tracer, said binder being a binder for at least the analyte, said tracer being comprised of a ligand labeled with a visible particulate label wherein when said particulate label is a sac including a visible dye, said sac is selected from the group consisting of liposomes and microcapsules, said ligand being bound to one of the binder on the support and the analyte bound to the binder on the support during an assay, said test area being formed of a material having a surface area for supporting the binder and the binder being supported in a concentration whereby bound tracer is visible on the support under assay condition, said binder being supported in a concentration of at least 1 μ

g/cm².

39. The kit of claim 38 wherein the test area is formed of nitrocellulose.

40. The kit of claim 39 wherein the binder is an antibody.

41. The kit of claim 40 wherein the label of the tracer is a lipsome including a visible dye.

42. The kit of claim 41 wherein the analyte is hCG.

43. The kit of claim 42 wherein the binder is antibody to hCG and the ligand of the tracer is antibody to hCG.

44. The kit of claim 41 wherein the analyte is digoxin, the binder is antibody to digoxin and the ligand of the tracer is an analogue of digoxin.

45. The kit of claim 39 wherein the label of the tracer is a liposome including a visible dye.

52. The kit of claim 38, 39 or 40 wherein the binder is in the test area as a spot.

53. The kit of claim 38, 39 or 40 wherein the particulate label is a precipitated or insoluble metal or metal alloy.

59. A process for assaying for an analyte, comprising:

contacting a binder for an analyte supported on a test area of a solid support with a solution of analyte to bind analyte to said binder;

determining bound analyte by use of a tracer comprised of a ligand labeled with a visible particulate label wherein when said particulate label is a sac including a visible dye said sac is selected from the group consisting of liposomes and microcapsules, said test area being formed of a material having a surface area for supporting the binder and the binder being supported in a concentration whereby tracer is visible on the support under assay conditions, said binder being supported in a concentration of at least 1 μ

g/cm², said analyte being determined by the visibility of the tracer in the test area.

60. The process of claim 59 wherein the test area is formed from nitrocellulose.

61. The process of claim 59 wherein the particulate label is a liposome including a visible dye.

62. The process of any one of claims 59, 60 or 61 wherein the binder is supported in a concentration of at least 10 ug/cm.