Blood partitioning apparatus

  • US 4,751,001 A
  • Filed: 09/24/1984
  • Issued: 06/14/1988
  • Est. Priority Date: 09/24/1984
  • Status: Expired due to Fees
First Claim
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1. A blood separation device suitable for the separation and partition of mononuclear cells from a blood sample, which comprises:

  • a centrifuge tube having a bottom closed end and an opposite top open end;

    a water soluble density gradient material disposed adjacent to the bottom closed end of the tube prior to centrifugation of the blood separation device; and

    a thixotropic gel-like substance disposed adjacent to and on top of the water soluble density gradient material prior to centrifugation of the blood separation device, the thixotropic gel-like substance acting as a partition between the water soluble density gradient material and a blood sample placed in the tube on top of the gel and acting to keep the water soluble density gradient material in place in the bottom of the tube and separated from the blood sample prior to centrifugation, the thixotropic gel being substantially hydrophobic, the water soluble density gradient material having a specific gravity which is higher than that of the thixotropic gel-like substance.

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