Cellular analysis system

• US 4,761,075 A
• Filed: 12/08/1986
• Issued: 08/02/1988
• Est. Priority Date: 12/10/1985
• Status: Expired due to Fees

• Current Assignee: Hitachi, Ltd.
• Original Assignee: Hitachi, Ltd.
• Inventors: Yokobayashi, Toshiaki, Matsushita, Hajime, Kurimura, Masaaki, Yabe, Ryohei
• Primary Examiner(s): LaRoche, Eugene R.
• Assistant Examiner(s): Pascal, Robert J.
• Application Number:
  US06/938,964
• Time in Patent Office: 603 Days
• Field of Search: 356/39, 356/72, 356/73, 356/432, 356/394, 414/331, 350/529
• US Class Current: 356/39
• CPC Class Codes: G01N 15/1433 using image recognition
• CPC Class Codes: G01N 15/1468 with spatial resolution of the texture or inner structure of the particle
• CPC Class Codes: G01N 2035/00039 Transport arrangements specific to flat sample substrates, e.g. pusher blade
• CPC Class Codes: G01N 2035/00089 Magazines
• CPC Class Codes: G01N 35/00029 provided with flat sample substrates, e.g. slides G01N35/028 takes precedence
• CPC Class Codes: G06V 20/69 Microscopic objects, e.g. biological cells or cellular parts

Abstract

An automatic cell analyzing system for automatically analyzing a large number of sample slides prepared for cellular samples. The sample slides include a plurality of sample slide sets each prepared by different staining methods for each sample. Cellular images obtained by observing through a microscope the numerous sample slides picked out sequentially are analyzed by an image fetching/feature extracting circuit, the resulting morphological features of the individual cells being converted into digital information to be stored. Upon completion of the analysis for all the sample slides, the digital information obtained from the sample slide is synthetically examined for each associated sample to thereby classify the sample into one of predetermined categories.

Claims

1. A cellular analysis system for automatically analyzing sample slides prepared for a number of samples, including sample slide sets each being prepared through predetermined different staining processes, comprising:

loading means for loading addressably and extractably said sample slides;

image forming means for automatically picking out said sample slides in sequence from said loading means to feed said slides into the field of view of a microscope to thereby form an image of at least one cell contained in said sample slide under observation of said microscope;

feature extracting means for extracting morphological features of said cell image as digital information by analyzing said cell image formed by said image forming means;

storage means for storing said digital information obtained through said feature extracting means together with a signal indicating an address of the associated sample slide in said loading means;

means for reading out said information derived from a set of the sample slides for each of the samples and stored in the storage means for thereby classifying said sample into one of plural predetermined categories on the basis of a comprehensive determination, wherein one of said categories indicates necessity for more accurate analysis, said sample slides associated with said samples being classified into said one of said categories being subjected to a secondary analysis, and wherein in said secondary analysis, said feature extracting means performs analysis for a greater number of cells than that of the cells analyzed in the primary analysis.

2. A cellular analysis system for automatically analyzing sample slides which are prepared by staining said samples using predetermined different staining processes and which are provided with staining marks indicative of the staining processes by which the samples of the slides are stained respectively, said system comprising:

loading means for loading addressably and extractably said sample slides;

memory means for preliminarily storing programs of analyzing procedures prepared for said respective staining processes;

means for transporting said sample slides successively from said loading means into a microscopic observation stage;

means for detecting said staining marks provided on said sample slides;

means responsive to the detection of said staining mark on each sample slide for selecting one of said programs stored in said memory means corresponding to said staining process indicated by said detected staining mark; and

means for applying a primary microscopic observation and analysis of the sample slide transported onto said microscopic stage according to said selected program, including means for forming an image of at least one cell contained on said sample slide under observation of a microscopic and feature extracting means for extracting morphological features of said image as digital information by analyzing said cell image according to said selected program.

3. A cellular analysis system according to claim 2, wherein each of said sample slides is marked by a sample code for identification of the particular sample on each sample slide, said system further comprising:

means for detecting said sample code marked on each of said sample slides;

storage means for storing said digital information obtained from each of said sample slides together with said detected sample code of each of said sample slides;

means for reading out of said storage means all said digital information stored therein together with said sample code for each corresponding one of said samples; and

means for determining abnormalities of each sample based on said read-out digital information according to a predetermined rule.

4. A cellular analysis system according to claim 3, wherein said determining means includes means for classifying each sample into one of the categories normal, abnormal and indefinite based on the results of said determination, said system further comprising means for applying a secondary analysis to said sample slides when a sample is classified into the indefinite category.

5. A cellular analysis system according to claim 4, wherein said primary analysis includes an analysis of a predetermined first number of cells contained in each sample slide and said secondary analysis includes a analysis of a predetermined second number of cells contained in each sample slide, said second number being larger than said first number.

6. A cellular analysis system according to claim 2, wherein said loading means includes cassettes for loading addressably and extractably only sample slides prepared by the same staining process, said staining mark indicating said staining process for said sample slides contained in each cassette being recorded on each corresponding cassette so as to be detectable by said staining mark detecting means.