Aqueous protein solutions stable to denaturation
First Claim
1. A method for stabilizing an aqueous solution of a water soluble biologically active protein to motion conditions, said solution having a pH between 6.8 and 8, which method comprises adding to said solution from 2 to 500 ppm of a surface active compound of the formula
whereinXn is a chain of n members selected from the group of members having the formulas ##STR7## in any sequence, n is 2 to 80,Y is --O-- or --NH--,R1 is --H, --CH3, or --C2 H5, and may be the same or different but is such that at least one-half of the members of the chain Xn contains --CH3 or --C2 H5, andR2 and R3 are each members independently selected from the group consisting of hydrogen, alkyl having 1 to 20 carbon atoms, carboxyalkyl having 2 to 20 carbon atoms, and alkylphenyl having 1 to 10 carbon atoms, but wherein R2 may only be such alkyl if Y is --NH--.
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Abstract
What are disclosed are a method for preventing the denaturation of proteins such as insulin in aqueous solution at interfaces by the addition to the solution of a surface-active substance comprising a chain of alternating weakly hydrophobic and weakly hydropholic zones, protein solutions containing such a surface-active substance, methods of purifying proteins contained in such solutions, and methods of treating surfaces with such a surface-active material to prevent the denaturation of proteins thereon.
97 Citations
8 Claims
- 1. A method for stabilizing an aqueous solution of a water soluble biologically active protein to motion conditions, said solution having a pH between 6.8 and 8, which method comprises adding to said solution from 2 to 500 ppm of a surface active compound of the formula
- space="preserve" listing-type="equation">R.sup.2 Y--X.sub.n --R.sup.3
wherein Xn is a chain of n members selected from the group of members having the formulas ##STR7## in any sequence, n is 2 to 80, Y is --O-- or --NH--, R1 is --H, --CH3, or --C2 H5, and may be the same or different but is such that at least one-half of the members of the chain Xn contains --CH3 or --C2 H5, and R2 and R3 are each members independently selected from the group consisting of hydrogen, alkyl having 1 to 20 carbon atoms, carboxyalkyl having 2 to 20 carbon atoms, and alkylphenyl having 1 to 10 carbon atoms, but wherein R2 may only be such alkyl if Y is --NH--. - View Dependent Claims (2)
- 3. A method for stabilizing an aqueous solution of a water soluble biologically active protein to motion conditions, said solution having a pH between 6.8 and 8, which method comprises adding to said solution from 2 to 200 ppm of a surface active compound of the formula
- space="preserve" listing-type="equation">R.sup.2 Y--X.sub.n --R.sup.3
wherein Xn is a chain of n members selected from the group of members having the formulas ##STR8## in any sequence, n is 2 to 80, Y is --O-- or --NH--, R1 is --H, --CH3, or --C2 H5, and may be the same or different but is such that at least one-half of the members of the chain Xn contains --CH3 or --C2 H5, and R2 and R3 are each members independently selected from the group consisting of hydrogen, alkyl having 1 to 20 carbon atoms, carboxyalkyl having 2 to 20 carbon atoms, and alkylphenyl having 1 to 10 carbon atoms, but wherein R2 may only be such alkyl if Y is --NH--. - View Dependent Claims (4, 5, 6, 7, 8)
Specification
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- Resources
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Current AssigneeSanofi-Aventis Deutschland GmbH (Sanofi )
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Original AssigneeHoechst Aktiengesellschaft (Sanofi )
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InventorsThurow, Horst
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Primary Examiner(s)Waddell, Frederick E.
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Application NumberUS06/564,346Time in Patent Office1,784 DaysField of Search514/2, 514/3US Class Current514/6.3CPC Class CodesA61K 38/00 Medicinal preparations cont...A61K 47/10 Alcohols; Phenols; Salts th...C07K 14/62 Insulins
