Micro sensor

• US 4,798,738 A
• Filed: 10/10/1986
• Issued: 01/17/1989
• Est. Priority Date: 10/10/1986
• Status: Expired due to Term

• Current Assignee: Terumo Cardiovascular Systems Corporation (Terumo Corporation)
• Original Assignee: Cardiovascular Decisions, LLC
• Inventors: Hui, Henry K., Yafuso, Masao
• Primary Examiner(s): BELL, JANYCE A
• Application Number:
  US06/917,913
• Time in Patent Office: 830 Days
• Field of Search: 427/163, 427/164, 427/165, 427/169, 427/2, 422/57, 422/56, 422/55, 436/169, 436/170, 436/172, 210/502.1
• US Class Current: 427/2.11
• CPC Class Codes: G01N 2021/6434 Optrodes
• CPC Class Codes: G01N 2021/6439 with indicators, stains, dyes, tags, labels, marks
• CPC Class Codes: G01N 21/643 non-biological material
• CPC Class Codes: G01N 21/80 Indicating pH value

Abstract

A micro pH sensor is constructed by reacting powdered aminoalkylated cellulose with a dye to covalently bond the dye to the aminoalkyl groups on the cellulose. Any excess unreacted aminoalkyl groups are blocked with a blocking agent and the dye containing powdered cellulose is taken up in a solvent. A portion of the solvated dye containing cellulose is deposited in association with an optical fiber. The cellulose is regenerated into a solid matrix by acid treatment of the solvated dye containing cellulose to deposit the dye containing cellulose matrix on the optical fiber.

Claims

1. A process of forming a micro sensor which comprises:

selecting a quantity of finely divided water insoluble ionic permeable hydrophylic polymer having a plurality of attachment sites thereon;

reacting said attachment sites on said polymer with a quantity of an event sensitive dye so as to attach said dye to at least some of said attachment sites on said polymer to form a dye bearing polymer;

dissolving said dye bearing polymer in a solvent;

depositing a quantity of said solvated dye bearing polymer onto an optical fiber; and

regenerating said dye bearing polymer from said solvent to form a solid matrix on said optical fiber of said regenerated dye bearing polymer.

2. The process of claim 1 further including:

selecting powdered cellulose as said polymer;

said attachment sites on said cellulose comprising substituent groups on said cellulose; and

attaching said dye to at least some of said substituent groups on said cellulose to form a dye substituted cellulose.

3. The process of claim 1 wherein:

said event sensitive dye is a pH sensitive dye and said micro sensor is a micro pH sensor.

4. The process of claim 1 further including:

selecting powdered cellulose as said polymer;

selecting said event sensitive dye as a pH sensitive dye and said micro sensor as a pH sensor;

said attachment sites on said cellulose comprising substituent groups on said cellulose; and

attaching said dye to said substituent groups on said cellulose by covalently bonding said dye to at least some of said substituent groups on cellulose.

5. The process of claim 4 further including:

reacting essentially any substituent groups on said cellulose not having said dye attached thereto with a blocking agent so as to render said blocked substituent groups essentially pH insensitive.

6. The process of claim 2 wherein:

said substituent groups on said cellulose comprises C₂ -C₂₀ aminoalkyl groups; and

said event sensitive dye is a pH sensitive dye and said micro sensor is a pH sensor.

7. The process of claim 6 further including:

attaching said dye to said aminoalkyl groups on said cellulose by covalently bonding said dye to at least some of said aminoalkyl substituents on said cellulose.

8. The process of claim 7 further including:

reacting essentially any remaining aminoalkyl groups on said cellulose not having said dye attached thereto with a blocking group so as to render said blocked aminoalkyl groups essentially pH insensitive.

9. The process of claim 8 wherein:

said remaining aminoalkyl groups are blocked by reacting with an acylating agent.

10. The process of claim 9 wherein:

said aminoalkyl groups are C₂ -C₈ ; and

said acylating agent is an acetylating agent.

11. The process of claim 2 wherein:

said dye is hydroxypyrenetrisulfonic acid or a salt thereof.

12. The process of claim 7 further including:

said dye being hydroxypyrenetrisulfonic acid or a salt thereof;

reacting said hydroxypyrenetrisulfonic acid to form a sulfonyl chloride derivative of said hydroxypyrenetrisulfonic acid; and

reacting said mono-sulfonyl chloride with said aminoalkyl cellulose to attach said dye to said cellulose by forming sulfonamide linkages between said dye and said aminoalkyl cellulose.

13. The process of claim 12 wherein:

said aminoalkyl cellulose is aminoethyl cellulose.

14. The process of claim 7 including:

adding a quantity of a permeability enhancing agent to said solution of said dye substituted cellulose.

15. The process of claim 14 including:

selecting glycerol as said permeability enhancing agent.

16. The process of claim 2 further including:

regenerating said dye substituted cellulose by treating with an acid said deposited quantity of said solvated dye substituted cellulose located on said optical fiber.

17. The process of claim 16 further including:

treating said optical fiber having said deposit of said solvated dye substituted cellulose located thereon by dipping said deposit of said solvated dye substituted cellulose on said optical fiber into an acid bath.

18. The process of claim 17 further including:

adding a quantity of glyceral to said acid bath prior to dipping said deposit of said solvated dye substituted cellulose located on said optical fiber into said acid bath.

19. A process of forming a pH sensor which comprises:

selecting a quantity of powdered cellulose having a plurality of substituent aminoalkyl groups thereon;

reacting said aminoalkyl groups on said cellulose with a quantity of a pH sensitive dye so as to covalently bond said dye to at least some of said aminoalkyl groups on said cellulose to form a dye substituted cellulose;

dissolving said dye substituted cellulose in a solvent;

depositing a quantity of said solvated dye substituted cellulose onto an optical fiber; and

regenerating said dye substituted cellulose from said solvent by acid treating said deposited quantity of said solvated dye substituted cellulose on said optical fiber to form a solid matrix of regenerated dye substituted cellulose on said optical fiber.

20. The process of claim 19 further including:

said dye being hydroxypyrenetrisulfonic acid or an acceptable salt thereof.

21. The process of claim 20 further including:

prior to dissolving said dye substituted cellulose in said solvent, reacting said dye substituted cellulose with an acylating agent so as to acylate any aminoalkyl groups which do not have said dye covalently bonded to them.

22. The process of claim 21 including:

selecting said aminoalkyl group from the group consisting of aminoethyl or aminopropyl.

23. The process of claim 19 further including:

coating said solid matrix of said regenerated dye substituted cellulose and a portion of said optical fiber surrounding said solid matrix with an overcoating of coating material.

24. The process of claim 23 wherein:

said overcoating material comprises cellulose having an opaque agent in said cellulose